Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)

doi:10.1371/journal.pone.0102492

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	Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)
	Wang, Qian1,2 ; Tan, Xungang1 ; Jiao, Shuang 1; You, Feng 1; Zhang, Pei-Jun 1; Tan, XG (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China.
	2014-07-24
发表期刊	PLOS ONE
卷号	9 期号:7 页码:e102492
文章类型	Article
摘要	Low temperatures may cause severe growth inhibition and mortality in fish. In order to understand the mechanism of cold tolerance, a transgenic zebrafish Tg (smyd1:m3ck) model was established to study the effect of energy homeostasis during cold stress. The muscle-specific promoter Smyd1 was used to express the carp muscle form III of creatine kinase (M3-CK), which maintained enzymatic activity at a relatively low temperature, in zebrafish skeletal muscle. In situ hybridization showed that M3-CK was expressed strongly in the skeletal muscle. When exposed to 13 degrees C, Tg (smyd1:m3ck) fish maintained their swimming behavior, while the wild-type could not. Energy measurements showed that the concentration of ATP increased in Tg (smyd1:m3ck) versus wild-type fish at 28 degrees C. After 2 h at 13 degrees C, ATP concentrations were 2.16-fold higher in Tg (smyd1:m3ck) than in wild-type (P<0.05). At 13 degrees C, the ATP concentration in Tg (smyd1:m3ck) fish and wild-type fish was 63.3% and 20.0%, respectively, of that in wild-type fish at 28 degrees C. Microarray analysis revealed differential expression of 1249 transcripts in Tg (smyd1:m3ck) versus wild-type fish under cold stress. Biological processes that were significantly overrepresented in this group included circadian rhythm, energy metabolism, lipid transport, and metabolism. These results are clues to understanding the mechanisms underlying temperature acclimation in fish.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0102492
收录类别	SCI
语种	英语
WOS记录号	WOS:000341354800022
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24071
专题	实验海洋生物学重点实验室海洋地质与环境重点实验室
通讯作者	Tan, XG (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Qian,Tan, Xungang,Jiao, Shuang,et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)[J]. PLOS ONE,2014,9(7):e102492.
APA	Wang, Qian,Tan, Xungang,Jiao, Shuang,You, Feng,Zhang, Pei-Jun,&Tan, XG .(2014).Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio).PLOS ONE,9(7),e102492.
MLA	Wang, Qian,et al."Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio)".PLOS ONE 9.7(2014):e102492.

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