Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri

doi:10.1016/j.fsi.2014.03.008

IOCAS-IR > 实验海洋生物学重点实验室

	Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri
	Shi, Xiaowei 1,2; Wang, Lingling 1; Zhou, Zhi 1; Liu, Rui 1; Li, Yunchen 2; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-05-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	38 期号:1 页码:204-210
文章类型	Article
摘要	Acetylcholine (ACh) is an indispensable neurotransmitter and neuromodulator in the cholinergic nervous system and it is implicated in the dynamic modulation of immune response in vertebrates. Although ACh has also been identified in most invertebrates, the knowledge about its immunomodulation is still limited. In the present study, the immunoreactivities of ACh and acetylcholinesterase (AChE) were observed in all the tested tissues of scallop Chlamys farreri, including adductor muscle, mantle, gill, hepatopancreas, kidney and gonad. The ACh concentration in the supernate of scallop hemolymph increased from 11.59 +/- 0.27 to 14.36 +/- 0.17 mu L-1 at 6 h after LPS (0.5 mg ml(-1)) stimulation, and increased to 15.51 +/- 1.20 mu M L-1 at 3 h after the stimulation of tumor necrosis factor alpha (TNF-alpha) (50 ng ml(-1)). After LPS stimulation, the mRNA expression levels of superoxide dismutase (CfSOD), catalase (CfCAT) and lysozyme (CfLYZ) in hemocytes increased significantly at 3 h (P < 0.05), 6 h (P < 0.05) and 12 h (P < 0.05), respectively. Compared with the LPS treatment, the induction of CfSOD, CfCAT and CfLYZ expression in hemocytes was repressed effectively (P < 0.05) by the co-stimulation of LPS and ACh (5 x 10(-7) M) at 3 h (P < 0.05), 6 h < 0.05) and 12 h (P < 0.05), respectively. Furthermore, the expression level of CfCAT in hemocytes increased significantly after 12 h by the co-stimulation with LPS and ACh (P < 0.05). These results indicated collectively that the scallop cholinergic nervous system could be activated by immune stimulations, and it might play an essential role in immunomodulation of scallops. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Acetylcholine (Ach) Lipopolysaccharide (Lps) Tumor Necrosis Factor Alpha (tnf-Alpha) Immunomodulation
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2014.03.008
收录类别	SCI
语种	英语
WOS记录号	WOS:000336353700027
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24065
专题	实验海洋生物学重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Linyi Univ, Coll Phys Educ, Linyi 276000, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Shi, Xiaowei,Wang, Lingling,Zhou, Zhi,et al. Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):204-210.
APA	Shi, Xiaowei.,Wang, Lingling.,Zhou, Zhi.,Liu, Rui.,Li, Yunchen.,...&Wang, LL .(2014).Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,38(1),204-210.
MLA	Shi, Xiaowei,et al."Acetylcholine modulates the immune response in Zhikong scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):204-210.

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