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| A low-density lipoprotein receptor-related protein (LRP)-like molecule identified from Chlamys farreri participated in immune response against bacterial infection | |
| Liu, Rui1; Wang, Lingling1; Sun, Ying1,2; Wang, Leilei1,2; Zhang, Huan1; Song, Linsheng1; Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2014-02-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 36期号:2页码:336-343 |
| 文章类型 | Article |
| 摘要 | Low-density lipoprotein receptor-related protein (LRP) is a group of important endocytic receptors contributing to binding ligands and maintaining internal environment. In the present study, an LRP-like molecule was identified from Zhikong scallop Chlamys farreri (CfLPR), and its mRNA expression profiles, tissue location, and immunology activities were analyzed to explore its possible function in the innate immune system. The ORF of CfLRP was of 1971 bp encoding a polypeptide of 656 amino acids with ten low-density lipoprotein-receptor YWTD (LY) domains and one scavenger receptor cysteine-rich (SRCR) domain. It shared similar structure with out-membrane domains of LRP family members in mammalian. The mRNA transcripts of CIRP were dominantly expressed in hepatopancreas and mantle (P < 0.01), and its mRNA level in hemocytes was up-regulated (P < 0.01) significantly after the stimulations of lipopolysaccharides (LPS), peptidoglycan (PGN) and beta-glucan. Western blotting assay using polyclonal antibody specific for CfLRP revealed that CfLRP was localized in the plasma. The recombinant protein of CfLRP (rCfLRP) could bind acetylated low density lipoprotein (Ac-LDL), metalloprotease SPF1 of Vibrio splendidus and mannan, but could not bind other typical PAMPs such as LPS, PGN, beta-glucan and zymosan. Meanwhile, rCfLRP also exhibited strong bacteriostatic activity to Gram-negative bacteria Vibrio anguillarum and V. splendidus. These results indicated that CfLRP could serve as a receptor to recognize and eliminate the invading pathogens, which provided a new implication in the function of LRP-like molecules in invertebrate immunity. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Chlamys Farreri Low-density Lipoprotein Receptor-related Protein (Lrp) Innate Immunity Ligands Binding Antimicrobial Activity |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.11.017 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332052100002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24059 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Rui,Wang, Lingling,Sun, Ying,et al. A low-density lipoprotein receptor-related protein (LRP)-like molecule identified from Chlamys farreri participated in immune response against bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(2):336-343. |
| APA | Liu, Rui.,Wang, Lingling.,Sun, Ying.,Wang, Leilei.,Zhang, Huan.,...&Song, LS .(2014).A low-density lipoprotein receptor-related protein (LRP)-like molecule identified from Chlamys farreri participated in immune response against bacterial infection.FISH & SHELLFISH IMMUNOLOGY,36(2),336-343. |
| MLA | Liu, Rui,et al."A low-density lipoprotein receptor-related protein (LRP)-like molecule identified from Chlamys farreri participated in immune response against bacterial infection".FISH & SHELLFISH IMMUNOLOGY 36.2(2014):336-343. |
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| A low-density lipopr（1595KB） | 限制开放 | ODC PDDL | 浏览 | |||
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