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| Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation | |
Zhao Ye1,2 ; Chen Muyan3; Wang Tianming4; Sun Lina1; Xu Dongxue1,2; Yang Hongsheng1; Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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| 2014-11-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 32期号:6页码:1248-1256 |
| 文章类型 | Article |
| 摘要 | Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a technique that is widely used for gene expression analysis, and its accuracy depends on the expression stability of the internal reference genes used as normalization factors. However, many applications of qRT-PCR used housekeeping genes as internal controls without validation. In this study, the expression stability of eight candidate reference genes in three tissues (intestine, respiratory tree, and muscle) of the sea cucumber Apostichopus japonicus was assessed during normal growth and aestivation using the geNorm, NormFinder, delta CT, and RefFinder algorithms. The results indicate that the reference genes exhibited significantly different expression patterns among the three tissues during aestivation. In general, the beta-tubulin (TUBB) gene was relatively stable in the intestine and respiratory tree tissues. The optimal reference gene combination for intestine was 40S ribosomal protein S18 (RPS18), TUBB, and NADH dehydrogenase (NADH); for respiratory tree, it was beta-actin (ACTB), TUBB, and succinate dehydrogenase cytochrome B small subunit (SDHC); and for muscle it was alpha-tubulin (TUBA) and NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (NDUFA13). These combinations of internal control genes should be considered for use in further studies of gene expression in A. japonicus during aestivation. |
| 关键词 | Apostichopus Japonicus Sea Cucumber Aestivation Quantitative Real-time Rt-pcr Reference Gene Normalization Factor |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-015-4004-2 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344541900004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24043 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Qingdao 266003, Peoples R China 4.Zhejiang Ocean Univ, Zhoushan 316022, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhao Ye,Chen Muyan,Wang Tianming,et al. Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(6):1248-1256. |
| APA | Zhao Ye.,Chen Muyan.,Wang Tianming.,Sun Lina.,Xu Dongxue.,...&Yang, HS .(2014).Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(6),1248-1256. |
| MLA | Zhao Ye,et al."Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.6(2014):1248-1256. |
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