Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| RNA-seq dependent transcriptional analysis unveils gene expression profile in the intestine of sea cucumber Apostichopus japonicus during aestivation | |
Zhao, Ye1,2 ; Yang, Hongsheng1; Storey, Kenneth B.3; Chen, Muyan4; Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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| 2014-06-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
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| 卷号 | 10页码:30-43 |
| 文章类型 | Article |
| 摘要 | The seasonal marine, the sea cucumber Apostichopus japonicus (Selenka, 1867), cycles annually between periods of torpor when water temperature is above about 25 degrees C in summer and active life when temperature is below about 18 degrees C. This species is a good candidate model organism for studies of environmentally-induced aestivation in marine invertebrates. Previous studies have examined various aspects of aestivation of A. japonicus, however, knowledge of the molecular regulation underpinning these events is still fragmentary. In the present study, we constructed a global gene expression profile of the intestine tissue of A. japonicus using RNA-seq to identify transcriptional responses associated with transitions between different states: non-aestivation (NA), deep-aestivation (DA), and arousal from aestivation (AA). The analysis identified 1245 differentially expressed genes (DEGs) between DA vs. NA states, 1338 DEGs between AA vs. DA, and 1321 DEGs between AA vs. NA using the criteria vertical bar Log(2)Ratio vertical bar >= 1 and FDR <= 0.001. Of these, 25 of the mostsignificant DEGs were verified by real-time PCR, showing trends in expression patterns that were almost in full concordance between the two techniques. GO analysis revealed that for DA vs. NA, 24 metabolic associated processes were highly enriched (corrected p value <0.05) whereas for AA vs. NA, 12 transport and metabolism associated processes were significantly enriched (corrected p value < 0.05). Pathways associated with aestivation were also mined, and indicated that most DEGs were enriched in metabolic and signal transduction pathways in the deep aestivation stage. Two up pathways were significantly enriched at the arousal stage (ribosome and metabolism of xenobiotics by cytochrome P450 pathway). A set of key DEGs was identified that may play vital roles in aestivation; these involved metabolism, detoxification and tissue protection, and energy-expensive processes. Our work presents an overview of dynamic gene expression in torpor arousal cycles during aestivation of A. japonicus and identifies a series of candidate genes and pathways for further research on the molecular mechanisms of aestivation. (C) 2014 Elsevier Inc All rights reserved. |
| 关键词 | Hypometabolism Differential Gene Expression Aestivation-arousal Cycles Echinoderm Environmental Stress |
| 学科领域 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| DOI | 10.1016/j.cbd.2014.02.002 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337931800004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24038 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Carleton Univ, Inst Biochem, Ottawa, ON K1S 5B6, Canada 4.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Ye,Yang, Hongsheng,Storey, Kenneth B.,et al. RNA-seq dependent transcriptional analysis unveils gene expression profile in the intestine of sea cucumber Apostichopus japonicus during aestivation[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2014,10:30-43. |
| APA | Zhao, Ye,Yang, Hongsheng,Storey, Kenneth B.,Chen, Muyan,&Yang, HS .(2014).RNA-seq dependent transcriptional analysis unveils gene expression profile in the intestine of sea cucumber Apostichopus japonicus during aestivation.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,10,30-43. |
| MLA | Zhao, Ye,et al."RNA-seq dependent transcriptional analysis unveils gene expression profile in the intestine of sea cucumber Apostichopus japonicus during aestivation".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 10(2014):30-43. |
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