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| Polymorphisms of heat shock protein 90 (Hsp90) in the sea cucumber Apostichopus japonicus and their association with heat-resistance | |
Xu, Dongxue1,2 ; Sun, Lina1; Liu, Shilin1; Zhang, Libin1; Yang, Hongsheng1; Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Nanhai Rd 7, Qingdao 266071, Shandong, Peoples R China.
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| 2014-12-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 41期号:2页码:428-436 |
| 文章类型 | Article |
| 摘要 | Heat shock protein 90 (Hsp90) functions as a molecular chaperone and plays an important role in the resistance of organisms to stress, particularly heat-stress. In our study, 12 exons and 11 introns of hsp90 were identified in the sea cucumber Aposticho pus japonicus. Twenty-two single nucleotide poly-morphisms (SNPs), including three non-synonymous mutations, were detected in the exons. Susceptible and resistant individuals were distinguished using a high-temperature (32 C) challenge experiment. Three blocks with high linkage disequilibrium were detected among these SNPs. Five of the twenty-two SNPs were shown to be significantly associated with susceptibility/resistance to high temperature by correlation analysis (chi-square test, P < 0.05). To confirm the importance of these five SNPs, a heat-resistance strain (HRS) was selected through three generations. Using the common population as the control group, it was shown that the distributions of genotypes and alleles of SNP e10-1 and ell-6 were significantly different between the two groups (P < 0.05). SNP e10-1 was trimorphic, with three alleles (A, C and T) and five genotypes (AA, CC, AT, CT and AC). The allele frequency of SNP e2-3 was also significantly associated with this trait (P < 0.05). This is the first demonstration of SNPs related to heat-resistance in A. japonicus and supports the use of SNP markers in the selective breeding of sea cucumbers. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Heat Shock Protein 90 Heat-resistance Snps Association Analysis Apostichopus Japonicus |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2014.09.025 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347017500038 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24034 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Nanhai Rd 7, Qingdao 266071, Shandong, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xu, Dongxue,Sun, Lina,Liu, Shilin,et al. Polymorphisms of heat shock protein 90 (Hsp90) in the sea cucumber Apostichopus japonicus and their association with heat-resistance[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):428-436. |
| APA | Xu, Dongxue,Sun, Lina,Liu, Shilin,Zhang, Libin,Yang, Hongsheng,&Yang, HS .(2014).Polymorphisms of heat shock protein 90 (Hsp90) in the sea cucumber Apostichopus japonicus and their association with heat-resistance.FISH & SHELLFISH IMMUNOLOGY,41(2),428-436. |
| MLA | Xu, Dongxue,et al."Polymorphisms of heat shock protein 90 (Hsp90) in the sea cucumber Apostichopus japonicus and their association with heat-resistance".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):428-436. |
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