Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense

doi:10.1371/journal.pone.0094030

	Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense
	Hu, Zhangxi1,2 ; Duan, Shunshan 2; Xu, Ning 2; Mulholland, Margaret R.3; Xu, N (reprint author), Jinan Univ, Res Ctr Hydrobiol, Guangzhou, Guangdong, Peoples R China.
	2014-04-07
发表期刊	PLOS ONE
卷号	9 期号:4 页码:e94030
文章类型	Article
摘要	We compared growth kinetics of Prorocentrum donghaiense cultures on different nitrogen (N) compounds including nitrate (NO3-), ammonium (NH4+), urea, glutamic acid (glu), dialanine (diala) and cyanate. P. donghaiense exhibited standard Monod-type growth kinetics over a range of N concentraions (0.5-500 mu mol NL-1 for NO3- and NH4+ , 0.5- 50 mu mol N L-1 for urea, 0.5-100 mu mol N L-1 for glu and cyanate, and 0.5-200 mu mol N L-1 for diala) for all of the N compounds tested. Cultures grown on glu and urea had the highest maximum growth rates (mu(m), 1.51=0.06 d(-1) and 1.50=0.05 d(-1), respectively). However, cultures grown on cyanate, NO3- , and NH4+ had lower half saturation constants (K-mu, 0.28-0.51 mu mol N L-1). N uptake kinetics were measured in NO3--deplete and -replete batch cultures of P. donghaiense. In NO3--deplete batch cultures, P. donghaiense exhibited Michaelis-Menten type uptake kinetics for NO3(-), NH4+, urea and algal amino acids; uptake was saturated at or below 50 mu mol N L-1. In NO3--replete batch cultures, NH4+, urea, and algal amino acid uptake kinetics were similar to those measured in NO3--deplete batch cultures. Together, our results demonstrate that P. donghaiense can grow well on a variety of N sources, and exhibits similar uptake kinetics under both nutrient replete and deplete conditions. This may be an important factor facilitating their growth during bloom initiation and development in N-enriched estuaries where many algae compete for bioavailable N and the nutrient environment changes as a result of algal growth.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0094030
收录类别	SCI
语种	英语
WOS记录号	WOS:000334159800090
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24018
专题	海洋生态与环境科学重点实验室
通讯作者	Xu, N (reprint author), Jinan Univ, Res Ctr Hydrobiol, Guangzhou, Guangdong, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Jinan Univ, Res Ctr Hydrobiol, Guangzhou, Guangdong, Peoples R China 3.Old Dominion Univ, Dept Ocean Earth & Atmospher Sci, Norfolk, VA USA
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Zhangxi,Duan, Shunshan,Xu, Ning,et al. Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense[J]. PLOS ONE,2014,9(4):e94030.
APA	Hu, Zhangxi,Duan, Shunshan,Xu, Ning,Mulholland, Margaret R.,&Xu, N .(2014).Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense.PLOS ONE,9(4),e94030.
MLA	Hu, Zhangxi,et al."Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense".PLOS ONE 9.4(2014):e94030.

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