Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus

doi:10.1007/s13131-014-0464-z

	Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus
	Ma Deyou 1,2; Yang Hongsheng1 ; Sun Lina 1,2; Xu Dongxue1,2 ; Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014-08-01
发表期刊	ACTA OCEANOLOGICA SINICA
卷号	33 期号:8 页码:55-61
文章类型	Article
摘要	The sea cucumber Apostichopus japonicus is an important economic species in China. Its dorsal body wall color is commonly tawny, whereas its ventral surface is fawn. Albino sea cucumbers are rarely observed. In order to profile gene expression and screen albinism-related genes, we compared the transcriptome of albino samples with a control by 454 cDNA sequencing. We found that 6 539 identified genes on the basis of sequence similarity to known genes were expressed in the albino A. japonicus. The gene ontology analysis indicated that the transcription of genes associated with the terms of biological regulation and pigmentation was non-abundant in the albino library compared to the control. Based on an analysis using the Kyoto Encyclopedia of Genes and Genomics (KEGG) database, we identified 14 important genes that were involved in major intercellular signaling pathways related to melanin synthesis, such as tyrosine metabolism, the mitogen-activated protein kinase (MAPK) pathway, and melanogenesis. The expressions of fibroblast growth factor receptor 4 (FGFR4), protein kinase C (PKC), protein kinase A (PKA), and Ras genes were significantly down-regulated in the albino transcriptome compared with the control, while the expressions of homogentisate 1, 2-dioxygenase gene (HGO), cAMP-responsive element binding protein (CREB), transcription factor AP-1(c-jun), and calmodulin (CaM) were significantly up-regulated (Fisher's exact test, p < 0.05). These differentially expressed genes could be candidate genes for revealing the mechanism of albinism and investigating regulation of melanin synthesis in A. japonicus.
关键词	454 Cdna Sequencing Albinism Apostichopus Japonicus Transcriptome
学科领域	Oceanography
DOI	10.1007/s13131-014-0464-z
收录类别	SCI
语种	英语
WOS记录号	WOS:000339318800007
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/23997
专题	海洋生态与环境科学重点实验室
通讯作者	Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ma Deyou,Yang Hongsheng,Sun Lina,et al. Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus[J]. ACTA OCEANOLOGICA SINICA,2014,33(8):55-61.
APA	Ma Deyou,Yang Hongsheng,Sun Lina,Xu Dongxue,&Yang, HS .(2014).Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus.ACTA OCEANOLOGICA SINICA,33(8),55-61.
MLA	Ma Deyou,et al."Comparative analysis of transcriptomes from albino and control sea cucumbers, Apostichopus japonicus".ACTA OCEANOLOGICA SINICA 33.8(2014):55-61.

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