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Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria
Qi, Peng1,2; Zhang, Dun1; Wan, Yi1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-08-01
Source PublicationELECTROANALYSIS
Volume26Issue:8Pages:1824-1830
SubtypeArticle
AbstractThis paper describes a novel electrochemical microbial biosensor based on Thiobacillus thioparus cells for sulfide detection. The morphology and electrochemical properties of the proposed biosensor were characterized by SEM and cyclic voltammetry, respectively. Working conditions of the microbial biosensor were optimized to obtain good electrochemical performances. Under the optimum conditions, analytical performances were evaluated, and the results suggested that the microbial biosensor could be used for selective detection of sulfide. The microbial biosensor was then successfully applied in detection of sulfate-reducing bacteria by oxidizing its characteristic metabolite, sulfide, which was accumulated in culture media during bacterial growth.
KeywordElectrochemical Biosensors Sulfate-reducing Bacteria Sulfide
Subject AreaChemistry ; Electrochemistry
DOI10.1002/elan.201400198
Indexed BySCI
Language英语
WOS IDWOS:000340557700022
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/23865
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorZhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Qi, Peng,Zhang, Dun,Wan, Yi,et al. Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria[J]. ELECTROANALYSIS,2014,26(8):1824-1830.
APA Qi, Peng,Zhang, Dun,Wan, Yi,&Zhang, D .(2014).Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria.ELECTROANALYSIS,26(8),1824-1830.
MLA Qi, Peng,et al."Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria".ELECTROANALYSIS 26.8(2014):1824-1830.
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