Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria

doi:10.1002/elan.201400198

	Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria
	Qi, Peng1,2 ; Zhang, Dun 1; Wan, Yi 1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2014-08-01
Source Publication	ELECTROANALYSIS
Volume	26 Issue:8 Pages:1824-1830
Subtype	Article
Abstract	This paper describes a novel electrochemical microbial biosensor based on Thiobacillus thioparus cells for sulfide detection. The morphology and electrochemical properties of the proposed biosensor were characterized by SEM and cyclic voltammetry, respectively. Working conditions of the microbial biosensor were optimized to obtain good electrochemical performances. Under the optimum conditions, analytical performances were evaluated, and the results suggested that the microbial biosensor could be used for selective detection of sulfide. The microbial biosensor was then successfully applied in detection of sulfate-reducing bacteria by oxidizing its characteristic metabolite, sulfide, which was accumulated in culture media during bacterial growth.
Keyword	Electrochemical Biosensors Sulfate-reducing Bacteria Sulfide
Subject Area	Chemistry ; Electrochemistry
DOI	10.1002/elan.201400198
Indexed By	SCI
Language	英语
WOS ID	WOS:000340557700022
Citation statistics	Cited Times:8[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.qdio.ac.cn/handle/337002/23865
Collection	海洋环境腐蚀与与生物污损重点实验室
Corresponding Author	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
Affiliation	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author Affilication	Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation GB/T 7714	Qi, Peng,Zhang, Dun,Wan, Yi,et al. Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria[J]. ELECTROANALYSIS,2014,26(8):1824-1830.
APA	Qi, Peng,Zhang, Dun,Wan, Yi,&Zhang, D .(2014).Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria.ELECTROANALYSIS,26(8),1824-1830.
MLA	Qi, Peng,et al."Development of an Amperometric Microbial Biosensor Based on Thiobacillus thioparus Cells for Sulfide and Its Application to Detection of Sulfate-Reducing Bacteria".ELECTROANALYSIS 26.8(2014):1824-1830.

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