A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity

doi:10.1016/j.talanta.2014.04.063

	A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity
	Qi, Peng1,2 ; Zhang, Dun 1; Wan, Yi 1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2014-11-01
发表期刊	TALANTA
卷号	129 页码:270-275
文章类型	Article
摘要	Sulfate-reducing bacteria (SRB) have been extensively studied in corrosion and environmental science. However, fast enumeration of SRB population is still a difficult task. This work presents a novel specific SRB detection method based on inhibition of cysteine protease activity. The hydrolytic activity of cysteine protease was inhibited by taking advantage of sulfide, the characteristic metabolic product of SRB, to attack active cysteine thiol group in cysteine protease catalytic sites. The active thiol S-sulfhydration process could be used for SRB detection, since the amount of sulfide accumulated in culture medium was highly related with initial bacterial concentration. The working conditions of cysteine protease have been optimized to obtain better detection capability, and the SRB detection performances have been evaluated in this work. The proposed SRB detection method based on inhibition of cysteine protease activity avoided the use of biological recognition elements. In addition, compared with the widely used most probable number (MPN) method which would take up to at least 15 days to accomplish whole detection process, the method based on inhibition of papain activity could detect SRB in 2 days, with a detection limit of 5.21 x 10(2) cfu mL(-1). The detection time for SRB population quantitative analysis was greatly shortened. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Sulfate-reducing Bacteria Cysteine Protease S-sulfhydration Inhibition
学科领域	Chemistry
DOI	10.1016/j.talanta.2014.04.063
收录类别	SCI
语种	英语
WOS记录号	WOS:000341463400038
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/23855
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Qi, Peng,Zhang, Dun,Wan, Yi,et al. A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity[J]. TALANTA,2014,129:270-275.
APA	Qi, Peng,Zhang, Dun,Wan, Yi,&Zhang, D .(2014).A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity.TALANTA,129,270-275.
MLA	Qi, Peng,et al."A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity".TALANTA 129(2014):270-275.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
A novel sulfate-redu（957KB）			限制开放	ODC PDDL	浏览