IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity
Qi, Peng1,2; Zhang, Dun1; Wan, Yi1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2014-11-01
Source PublicationTALANTA
Volume129Pages:270-275
SubtypeArticle
AbstractSulfate-reducing bacteria (SRB) have been extensively studied in corrosion and environmental science. However, fast enumeration of SRB population is still a difficult task. This work presents a novel specific SRB detection method based on inhibition of cysteine protease activity. The hydrolytic activity of cysteine protease was inhibited by taking advantage of sulfide, the characteristic metabolic product of SRB, to attack active cysteine thiol group in cysteine protease catalytic sites. The active thiol S-sulfhydration process could be used for SRB detection, since the amount of sulfide accumulated in culture medium was highly related with initial bacterial concentration. The working conditions of cysteine protease have been optimized to obtain better detection capability, and the SRB detection performances have been evaluated in this work. The proposed SRB detection method based on inhibition of cysteine protease activity avoided the use of biological recognition elements. In addition, compared with the widely used most probable number (MPN) method which would take up to at least 15 days to accomplish whole detection process, the method based on inhibition of papain activity could detect SRB in 2 days, with a detection limit of 5.21 x 10(2) cfu mL(-1). The detection time for SRB population quantitative analysis was greatly shortened. (C) 2014 Elsevier B.V. All rights reserved.
KeywordSulfate-reducing Bacteria Cysteine Protease S-sulfhydration Inhibition
Subject AreaChemistry
DOI10.1016/j.talanta.2014.04.063
Indexed BySCI
Language英语
WOS IDWOS:000341463400038
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/23855
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorZhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Qi, Peng,Zhang, Dun,Wan, Yi,et al. A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity[J]. TALANTA,2014,129:270-275.
APA Qi, Peng,Zhang, Dun,Wan, Yi,&Zhang, D .(2014).A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity.TALANTA,129,270-275.
MLA Qi, Peng,et al."A novel sulfate-reducing bacteria detection method based on inhibition of cysteine protease activity".TALANTA 129(2014):270-275.
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