| A close phylogenetic relationship between Sipuncula and Annelida evidenced from the complete mitochondrial genome sequence of Phascolosoma esculenta | |
| Shen, Xin1,2; Ma, Xiaoyin3; Ren, Jianfeng2; Zhao, Fangqing4; Shen, X, Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China | |
| 2009-03-28 | |
| 发表期刊 | BMC GENOMICS
 |
| ISSN | 1471-2164 |
| 卷号 | 10页码:136 |
| 文章类型 | Article |
| 摘要 | Background: There are many advantages to the application of complete mitochondrial (mt) genomes in the accurate reconstruction of phylogenetic relationships in Metazoa. Although over one thousand metazoan genomes have been sequenced, the taxonomic sampling is highly biased, left with many phyla without a single representative of complete mitochondrial genome. Sipuncula (peanut worms or star worms) is a small taxon of worm-like marine organisms with an uncertain phylogenetic position. In this report, we present the mitochondrial genome sequence of Phascolosoma esculenta, the first complete mitochondrial genome of the phylum.; Background: There are many advantages to the application of complete mitochondrial (mt) genomes in the accurate reconstruction of phylogenetic relationships in Metazoa. Although over one thousand metazoan genomes have been sequenced, the taxonomic sampling is highly biased, left with many phyla without a single representative of complete mitochondrial genome. Sipuncula (peanut worms or star worms) is a small taxon of worm-like marine organisms with an uncertain phylogenetic position. In this report, we present the mitochondrial genome sequence of Phascolosoma esculenta, the first complete mitochondrial genome of the phylum. Results: The mitochondrial genome of P. esculenta is 15,494 bp in length. The coding strand consists of 32.1% A, 21.5% C, 13.0% G, and 33.4% T bases (AT = 65.5%; AT skew = -0.019; GC skew = -0.248). It contains thirteen protein-coding genes (PCGs) with 3,709 codons in total, twenty-two transfer RNA genes, two ribosomal RNA genes and a non-coding AT-rich region (AT = 74.2%). All of the 37 identified genes are transcribed from the same DNA strand. Compared with the typical set of metazoan mt genomes, sipunculid lacks trnR but has an additional trnM. Maximum Likelihood and Bayesian analyses of the protein sequences show that Myzostomida, Sipuncula and Annelida (including echiurans and pogonophorans) form a monophyletic group, which supports a closer relationship between Sipuncula and Annelida than with Mollusca, Brachiopoda, and some other lophotrochozoan groups. Conclusion: This is the first report of a complete mitochondrial genome as a representative within the phylum Sipuncula. It shares many more similar features with the four known annelid and one echiuran mtDNAs. Firstly, sipunculans and annelids share quite similar gene order in the mitochondrial genome, with all 37 genes located on the same strand; secondly, phylogenetic analyses based on the concatenated protein sequences also strongly support the sipunculan + annelid clade (including echiurans and pogonophorans). Hence annelid "key-characters" including segmentation may be more labile than previously assumed. |
| 关键词 | Gene Order Developmental Stages Nervous-system Urechis-caupo Sister Taxa Echiura Evolution Morphology Arthropoda Mollusca |
| 学科领域 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| DOI | 10.1186/1471-2164-10-136 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265793100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/1872 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Shen, X, Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China |
| 作者单位 | 1.Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Wenzhou Med Coll, Wenzhou 325035, Peoples R China 4.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Shen, Xin,Ma, Xiaoyin,Ren, Jianfeng,et al. A close phylogenetic relationship between Sipuncula and Annelida evidenced from the complete mitochondrial genome sequence of Phascolosoma esculenta[J]. BMC GENOMICS,2009,10:136. |
| APA | Shen, Xin,Ma, Xiaoyin,Ren, Jianfeng,Zhao, Fangqing,&Shen, X, Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China.(2009).A close phylogenetic relationship between Sipuncula and Annelida evidenced from the complete mitochondrial genome sequence of Phascolosoma esculenta.BMC GENOMICS,10,136. |
| MLA | Shen, Xin,et al."A close phylogenetic relationship between Sipuncula and Annelida evidenced from the complete mitochondrial genome sequence of Phascolosoma esculenta".BMC GENOMICS 10(2009):136. |
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