| Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready) | |
| Ran, Tao1,2; Mei, Liu1; Lei, Wang1; Aihua, Liu3; Ru, He4; Jie, Sun1; Mei, L, Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, 7th Nanhai Rd, Qingdao 266071, Peoples R China | |
| 2009-08-20 | |
| 发表期刊 | AQUACULTURE RESEARCH
 |
| ISSN | 1355-557X |
| 卷号 | 40期号:12页码:1350-1357 |
| 文章类型 | Article |
| 摘要 | We used nested-polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10(-10) g mu L(-1) DNA solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous DNA segments. We also compared genetically modified (GM) soybean with non-GM soybean diets in feeding tilapias (Oreochromis niloticus, GIFT strain) and examined the residual fragments (254 bp) of GM soybeans. Tilapias receiving GM soybean diets had DNA fragments in different tissues and organs, indicating that exogenous GM genes were absorbed systemically and not completely degraded by the tilapia's alimentary canal.; We used nested-polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10(-10) g mu L-1 DNA solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous DNA segments. We also compared genetically modified (GM) soybean with non-GM soybean diets in feeding tilapias (Oreochromis niloticus, GIFT strain) and examined the residual fragments (254 bp) of GM soybeans. Tilapias receiving GM soybean diets had DNA fragments in different tissues and organs, indicating that exogenous GM genes were absorbed systemically and not completely degraded by the tilapia's alimentary canal. |
| 关键词 | Aquatic Feed Gm Soybean Nested-pcr Oreochromis Niloticus Tissues Transfer |
| 学科领域 | Fisheries |
| DOI | 10.1111/j.1365-2109.2009.02187.x |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269056300002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/1868 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Mei, L, Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, 7th Nanhai Rd, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Rongcheng Entry Exit Inspect & Quarantine Bur, Rongcheng, Peoples R China 3.Qingdao Entry Exit Inspect & Quarantine Bur, Qingdao, Peoples R China 4.Haerbin Inst Technol, Weihai, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Ran, Tao,Mei, Liu,Lei, Wang,et al. Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)[J]. AQUACULTURE RESEARCH,2009,40(12):1350-1357. |
| APA | Ran, Tao.,Mei, Liu.,Lei, Wang.,Aihua, Liu.,Ru, He.,...&Mei, L, Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, 7th Nanhai Rd, Qingdao 266071, Peoples R China.(2009).Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready).AQUACULTURE RESEARCH,40(12),1350-1357. |
| MLA | Ran, Tao,et al."Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)".AQUACULTURE RESEARCH 40.12(2009):1350-1357. |
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