LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus

doi:10.24272/j.issn.2095-8137.2022.520

IOCAS-IR > 实验海洋生物学重点实验室

	LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus
	Ning, Xian-Hui 1,2; Han, Bing 1; Peng, Ye 1; Yin, Shao-Wu 1,3
	2024-01-18
发表期刊	ZOOLOGICAL RESEARCH
ISSN	2095-8137
卷号	45 期号:1 页码:25-35
通讯作者	Yin, Shao-Wu(yinshaowu@163.com)
摘要	Long non-coding RNAs (lncRNAs) function as key modulators in mammalian immunity, particularly due to their involvement in lncRNA-mediated competitive endogenous RNA (ceRNA) crosstalk. Despite their recognized significance in mammals, research on lncRNAs in lower vertebrates remains limited. In the present study, we characterized the first immune-related lncRNA (pol-lnc78) in the teleost Japanese flounder (Paralichthys olivaceus). Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein (SARM), the fifth discovered member of the Toll/interleukin 1 (IL-1) receptor (TIR) adaptor family. This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor (TLR) signaling pathway. Specifically, SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1 beta and tumor necrosis factor-alpha (TNF-alpha). Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3' untranslated region (UTR), thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination. Furthermore, pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM expression. During infection, the negative regulators pol-lnc78 and SARM were significantly down-regulated, while pol-miR-n199-3p was significantly up-regulated, thus favoring host antibacterial defense. These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.
关键词	LncRNA SARM miRNA ceRNA Antibacterial response
DOI	10.24272/j.issn.2095-8137.2022.520
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42006082] ; Natural Science Foundation of Jiangsu Province of China[BK20221323] ; JBGS Project of Seed Industry Revitalization in Jiangsu Province[JBGS [2021] 034] ; State Key Laboratory of Developmental Biology of Freshwater Fish[2021KF009]
WOS研究方向	Zoology
WOS类目	Zoology
WOS记录号	WOS:001165342100002
出版者	SCIENCE PRESS
WOS关键词	LONG NONCODING RNA ; VIBRIO-ANGUILLARUM ; MOLECULAR CHARACTERIZATION ; CAENORHABDITIS-ELEGANS ; TRANSCRIPTOME ANALYSIS ; IMMUNE-RESPONSE ; INNATE IMMUNITY ; MESSENGER-RNA ; PROTEIN ; MIRNAS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184602
专题	实验海洋生物学重点实验室
通讯作者	Yin, Shao-Wu
作者单位	1.Nanjing Normal Univ, Coll Marine Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Coinnovat Ctr Marine Bioind Technol Jiangsu Prov, Lianyungang 222005, Jiangsu, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ning, Xian-Hui,Han, Bing,Peng, Ye,et al. LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus[J]. ZOOLOGICAL RESEARCH,2024,45(1):25-35.
APA	Ning, Xian-Hui,Han, Bing,Peng, Ye,&Yin, Shao-Wu.(2024).LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus.ZOOLOGICAL RESEARCH,45(1),25-35.
MLA	Ning, Xian-Hui,et al."LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus".ZOOLOGICAL RESEARCH 45.1(2024):25-35.