Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea <i>Planctomycetes</i> bacteria

doi:10.7554/eLife.89874

IOCAS-IR > 实验海洋生物学重点实验室

	*Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes* bacteria**
	Zheng, Rikuan1,2,3,4,5 ; Wang, Chong 1,2,3,4,5; Liu, Rui 1,2,3,4,5; Cai, Ruining 1,2,3,4,5,6; Sun, Chaomin 1,2,3,4,5,6
	2024-01-24
发表期刊	ELIFE
ISSN	2050-084X
卷号	12 页码:23
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
摘要	Planctomycetes bacteria are ubiquitously distributed across various biospheres and play key roles in global element cycles. However, few deep-sea Planctomycetes members have been cultivated, limiting our understanding of Planctomycetes in the deep biosphere. Here, we have successfully cultured a novel strain of Planctomycetes (strain ZRK32) from a deep-sea cold seep sediment. Our genomic, physiological, and phylogenetic analyses indicate that strain ZRK32 is a novel species, which we propose be named: Poriferisphaera heterotrophicis. We show that strain ZRK32 replicates using a budding mode of division. Based on the combined results from growth assays and transcriptomic analyses, we found that rich nutrients, or supplementation with NO(3)(- )or NH4+ promoted the growth of strain ZRK32 by facilitating energy production through the tricarboxylic acid cycle and the Embden-Meyerhof-Parnas glycolysis pathway. Moreover, supplementation with NO(3)(- )or NH4+ induced strain ZRK32 to release a bacteriophage in a chronic manner, without host cell lysis. This bacteriophage then enabled strain ZRK32, and another marine bacterium that we studied, to metabolize nitrogen through the function of auxiliary metabolic genes. Overall, these findings expand our understanding of deep-sea Planctomycetes bacteria, while highlighting their ability to metabolize nitrogen when reprogrammed by chronic viruses.
关键词	Planctomycetes deep sea cultivation nitrogen metabolism bacteriophages Other
DOI	10.7554/eLife.89874
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[92051107] ; National Natural Science Foundation of China[92351301] ; National Natural Science Foundation of China[42221005] ; Laoshan Laboratory[LSKJ202203103] ; Chaomin Sun Laoshan Laboratory[2022QNLM030004-3] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Chinese Academy of Sciences[XDA22050301] ; China Ocean Mineral Resources RD Association[DY135-B2-14] ; Alliance of International Science Organizations[ANSO-CR-KP-2022-08] ; Center for Ocean MegaScience, Chinese Academy of Sciences[COMS2020Q04] ; Natural Science Foundation of Shandong Province[tstp20230637]
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:001150769400001
出版者	eLIFE SCIENCES PUBL LTD
WOS关键词	GEN. NOV. ; FAM. NOV. ; CLASS PHYCISPHAERAE ; SP. NOV. ; PROTEIN ; DIVERSITY ; PROPOSAL ; LIFE ; TOOL ; ECOLOGY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184584
专题	实验海洋生物学重点实验室深海极端环境与生命过程研究中心
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zheng, Rikuan,Wang, Chong,Liu, Rui,et al. Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes bacteria[J]. ELIFE,2024,12:23.
APA	Zheng, Rikuan,Wang, Chong,Liu, Rui,Cai, Ruining,&Sun, Chaomin.(2024).Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes bacteria.ELIFE,12,23.
MLA	Zheng, Rikuan,et al."Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes bacteria".ELIFE 12(2024):23.