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A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates
Gu, Xiaoqian1,2; Fu, Liping1; Wang, Zhiyan1; Cao, Zhe1; Zhao, Luying1; Seswita-Zilda, Dewi3; Zhang, Ao4; Zhang, Qian1; Li, Jiang1
2024-02-19
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
卷号72期号:8页码:4116-4126
通讯作者Li, Jiang(lijiang@fio.org.cn)
摘要Alginate lyase Aly448, a potential new member of the polysaccharide lyase (PL) 7 family, which was cloned and identified from the macroalgae-associated bacterial metagenomic library, showed bifunctionality. The molecular docking results revealed that Aly448 has two completely different binding sites for alginate (polyMG), poly-alpha-l-guluronic acid (polyG), and poly-beta-d-mannuronic acid (polyM) substrates, respectively, which might be the molecular basis for the enzyme's bifunctionality. Truncational results confirmed that predicted key residues affected the bifunctionality of Aly448, but did not wholly explain. Besides, Aly448 presented excellent biochemical characteristics, such as higher thermal stability and pH tolerance. Degradation of polyMG, polyM, and polyG substrates by Aly448 produced tetrasaccharide (DP4), disaccharide (DP2), and galactose (DP1), which exhibited excellent antioxidant activity. These findings provide novel insights into the substrate recognition mechanism of bifunctional alginate lyases and pave a new path for the exploitation of natural antioxidant agents.
关键词bifunctional alginatelyase alginate oligosaccharides antioxidant activity
DOI10.1021/acs.jafc.3c08638
收录类别SCI
语种英语
资助项目Impact and Response of Antarctic Seas to Climate Change[IRFSOCC2023-2025] ; Impact and Response of Antarctic Seas to Climate Change
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
WOS类目Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号WOS:001166898100001
出版者AMER CHEMICAL SOC
WOS关键词PURIFICATION ; OLIGOSACCHARIDES ; PL-7
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/184488
专题实验海洋生物学重点实验室
通讯作者Li, Jiang
作者单位1.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Natl Res & Innovat Agcy BRIN, Res Ctr Deep Sea Earth Sci & Maritime Res Org, Jakarta 14430, Indonesia
4.Qingdao Univ Sci & Technol, Chem Engn Inst, Qingdao 266042, Peoples R China
第一作者单位中国科学院海洋研究所
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Gu, Xiaoqian,Fu, Liping,Wang, Zhiyan,et al. A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(8):4116-4126.
APA Gu, Xiaoqian.,Fu, Liping.,Wang, Zhiyan.,Cao, Zhe.,Zhao, Luying.,...&Li, Jiang.(2024).A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(8),4116-4126.
MLA Gu, Xiaoqian,et al."A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.8(2024):4116-4126.
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