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A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates
Gu, Xiaoqian1,2; Fu, Liping1; Wang, Zhiyan1; Cao, Zhe1; Zhao, Luying1; Seswita-Zilda, Dewi3; Zhang, Ao4; Zhang, Qian1; Li, Jiang1
2024-02-19
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume72Issue:8Pages:4116-4126
Corresponding AuthorLi, Jiang(lijiang@fio.org.cn)
AbstractAlginate lyase Aly448, a potential new member of the polysaccharide lyase (PL) 7 family, which was cloned and identified from the macroalgae-associated bacterial metagenomic library, showed bifunctionality. The molecular docking results revealed that Aly448 has two completely different binding sites for alginate (polyMG), poly-alpha-l-guluronic acid (polyG), and poly-beta-d-mannuronic acid (polyM) substrates, respectively, which might be the molecular basis for the enzyme's bifunctionality. Truncational results confirmed that predicted key residues affected the bifunctionality of Aly448, but did not wholly explain. Besides, Aly448 presented excellent biochemical characteristics, such as higher thermal stability and pH tolerance. Degradation of polyMG, polyM, and polyG substrates by Aly448 produced tetrasaccharide (DP4), disaccharide (DP2), and galactose (DP1), which exhibited excellent antioxidant activity. These findings provide novel insights into the substrate recognition mechanism of bifunctional alginate lyases and pave a new path for the exploitation of natural antioxidant agents.
Keywordbifunctional alginatelyase alginate oligosaccharides antioxidant activity
DOI10.1021/acs.jafc.3c08638
Indexed BySCI
Language英语
Funding ProjectImpact and Response of Antarctic Seas to Climate Change[IRFSOCC2023-2025] ; Impact and Response of Antarctic Seas to Climate Change
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:001166898100001
PublisherAMER CHEMICAL SOC
WOS KeywordPURIFICATION ; OLIGOSACCHARIDES ; PL-7
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/184488
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Jiang
Affiliation1.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Natl Res & Innovat Agcy BRIN, Res Ctr Deep Sea Earth Sci & Maritime Res Org, Jakarta 14430, Indonesia
4.Qingdao Univ Sci & Technol, Chem Engn Inst, Qingdao 266042, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gu, Xiaoqian,Fu, Liping,Wang, Zhiyan,et al. A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(8):4116-4126.
APA Gu, Xiaoqian.,Fu, Liping.,Wang, Zhiyan.,Cao, Zhe.,Zhao, Luying.,...&Li, Jiang.(2024).A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(8),4116-4126.
MLA Gu, Xiaoqian,et al."A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.8(2024):4116-4126.
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