Bioleaching of Chalcopyrite by a New Strain <i>Leptospirillum ferrodiazotrophum</i> Ksh-L Isolated from a Dump-Bioleaching System of Kashen Copper-Molybdenum Mine

doi:10.3390/min14010026

	*Bioleaching of Chalcopyrite by a New Strain Leptospirillum ferrodiazotrophum* Ksh-L Isolated from a Dump-Bioleaching System of Kashen Copper-Molybdenum Mine**
	Khachatryan, Anna 1; Vardanyan, Narine 1; Willscher, Sabine 2; Sevoyan, Garegin 3; Zhang, Ruiyong 4; Vardanyan, Arevik 1
	2024
发表期刊	MINERALS
卷号	14 期号:1 页码:18
通讯作者	Zhang, Ruiyong(ruiyong.zhang@qdio.ac.cn) ; Vardanyan, Arevik(arevik.vardanyan@asnet.am)
摘要	A new strain of Leptospirillum sp. Ksh-L was isolated from a dump-bioleaching system of the Kashen copper-molybdenum mine (South Caucasus). Ksh-L is an obligate chemolithoautotroph, capable of oxidizing ferrous iron (Fe2+). Cells are Gram-negative and vibrio- or spirillum-shaped of a 0.5-3 mu m size. The optimal conditions for the growth are 35 degrees C and pH 1.6-1.8. Cu2+ and Zn2+ have different effects on the oxidizing ability of the Leptospirillum sp. Ksh-L culture depending on the phase of growth and concentration of Fe2+. Under the conditions of gradually increasing the concentration of copper in the medium, during 4-5 successive subculturing experiments, it was possible to obtain an adapted culture of Leptospirillum sp. Ksh-L, capable of growing in the medium in the presence of up to 400 mM Cu2+. A bioleaching experiment indicates that Ksh-L can efficiently oxidize chalcopyrite. However, the bioleaching of copper from chalcopyrite by Leptospirillum ferrodiazotropum Ksh-L increased about 1.8 times in association with At. thiooxidans ATCC 19377. Phylogenetic analysis based on 16S rRNA gene sequences (GenBank ID ON226845) shows that strain Ksh-L forms a single cluster into Group III. The strain possesses 99.59%, 99.52%, and 96.60% sequence similarity with the strains YTW-96-06, YTW-66-06, and Leptospirillum ferrodiazotrophum 5C in Group III, respectively.
关键词	Leptospirillum ferrodiazotrophum isolation characterization phylogenetic analysis bioleaching of sulfide minerals
DOI	10.3390/min14010026
收录类别	SCI
语种	英语
资助项目	Higher Education Science Committee of the Ministry of Education, Science, Culture and Sports of Republic of the Armenia
WOS研究方向	Geochemistry & Geophysics ; Mineralogy ; Mining & Mineral Processing
WOS类目	Geochemistry & Geophysics ; Mineralogy ; Mining & Mineral Processing
WOS记录号	WOS:001152423900001
出版者	MDPI
WOS关键词	SP-NOV ; ACIDITHIOBACILLUS-FERROOXIDANS ; THIOBACILLUS-FERROOXIDANS ; OXIDIZING BACTERIUM ; MIXED CULTURES ; RIBOSOMAL-RNA ; IRON ; FERRIPHILUM ; MECHANISMS ; TOLERANCE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184429
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, Ruiyong; Vardanyan, Arevik
作者单位	1.SPC Armbiotechnol NAS Armenia, Inst Microbiol, Lab Met Bioleaching, 14 Gyurjyan Str, Yerevan 0056, Armenia 2.Martin Luther Univ Halle Wittenberg, Fac Nat Sci 1, Hoher Weg 8, D-06120 Halle, Germany 3.Yerevan State Univ, Fac Biol, Dept Ecol, 1 Alex Manoogian Str, Yerevan 0025, Armenia 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Khachatryan, Anna,Vardanyan, Narine,Willscher, Sabine,et al. Bioleaching of Chalcopyrite by a New Strain Leptospirillum ferrodiazotrophum Ksh-L Isolated from a Dump-Bioleaching System of Kashen Copper-Molybdenum Mine[J]. MINERALS,2024,14(1):18.
APA	Khachatryan, Anna,Vardanyan, Narine,Willscher, Sabine,Sevoyan, Garegin,Zhang, Ruiyong,&Vardanyan, Arevik.(2024).Bioleaching of Chalcopyrite by a New Strain Leptospirillum ferrodiazotrophum Ksh-L Isolated from a Dump-Bioleaching System of Kashen Copper-Molybdenum Mine.MINERALS,14(1),18.
MLA	Khachatryan, Anna,et al."Bioleaching of Chalcopyrite by a New Strain Leptospirillum ferrodiazotrophum Ksh-L Isolated from a Dump-Bioleaching System of Kashen Copper-Molybdenum Mine".MINERALS 14.1(2024):18.