Novel porphyrin derivative containing cations as new photodynamic antimicrobial agent with high efficiency

doi:10.1039/d3ra07743h

	Novel porphyrin derivative containing cations as new photodynamic antimicrobial agent with high efficiency
	Zhang, Jiajing 1,2,3; Yuan, Xiaoqian 3; Li, Hongsen 3; Yu, Liting 3; Zhang, Yulong 3; Pang, Keyi 3; Sun, Chaoyue 3; Liu, Zhongyang 3; Li, Jie3 ; Ma, Liying 3; Song, Jinming1,4 ; Chen, Lingxin 2,3
	2024-01-17
发表期刊	RSC ADVANCES
卷号	14 期号:5 页码:3122-3134
通讯作者	Ma, Liying() ; Chen, Lingxin(lxchen@yic.ac.cn)
摘要	Bacterial infections from chronic wounds affect about 175 million people each year and are a significant clinical problem. Through the integration of photodynamic therapy (PDT) and chemotherapy, a new photosensitizer consisting of ammonium salt N,N-bis-(2-hydroxyethyl)-N-(6-(4-(10,15,20-trimesitylporphyrin-5-yl) phenoxy) hexane)-N-methanaminium bromide, TMP(+) was successfully synthesized with a total reaction yield of 10%. The novel photosensitizer consists of two parts, a porphyrin photosensitizer part and a quaternary ammonium salt part, to achieve the synergistic effect of photodynamic and chemical antibacterial activity. With the increase of TMP(+) concentration, the diameter of the PCT fiber membranes (POL/COL/TMP(+); POL, polycaprolactone; COL, collagen) gradually increased, which was caused by the charge of the quaternary ammonium salt. At the same time, the antibacterial properties were gradually improved. We finally selected the PCT 0.5% group for the antibacterial experiment, with excellent performance in fiber uniformity, hydrophobicity and biosafety. The antibacterial experiment showed that the modified porphyrin TMP(+) had a better antibacterial effect than others. In vivo chronic wound healing experiments proved that the antibacterial and anti-inflammatory effect of the PCTL group was the best, further confirmed by H&E histological analysis, immunofluorescence and immunohistochemistry mechanism experiments. This research lays the foundation for the manufacture of novel molecules that combine chemical and photodynamic strategies. A new photosensitizer consisting of ammonium salt, TMP(+) was synthesized and achieved the synergistic effect of photodynamic and chemical antibacterial activity. Experiments showed promising bactericidal properties, while accelerating the healing of Staphylococcus aureus infected mice.
DOI	10.1039/d3ra07743h
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[22001020] ; Foundation of Binzhou Medical University[2019KYQD23] ; Foundation of Binzhou Medical University[2022KYQD27]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001144849200001
出版者	ROYAL SOC CHEMISTRY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184273
专题	海洋生态与环境科学重点实验室
通讯作者	Ma, Liying; Chen, Lingxin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Zhang, Jiajing,Yuan, Xiaoqian,Li, Hongsen,et al. Novel porphyrin derivative containing cations as new photodynamic antimicrobial agent with high efficiency[J]. RSC ADVANCES,2024,14(5):3122-3134.
APA	Zhang, Jiajing.,Yuan, Xiaoqian.,Li, Hongsen.,Yu, Liting.,Zhang, Yulong.,...&Chen, Lingxin.(2024).Novel porphyrin derivative containing cations as new photodynamic antimicrobial agent with high efficiency.RSC ADVANCES,14(5),3122-3134.
MLA	Zhang, Jiajing,et al."Novel porphyrin derivative containing cations as new photodynamic antimicrobial agent with high efficiency".RSC ADVANCES 14.5(2024):3122-3134.