Ultrasensitive fluorescence detection of gonyautoxins in seawater using a novel molecularly imprinted nanoprobe

doi:10.1016/j.scitotenv.2023.169056

	Ultrasensitive fluorescence detection of gonyautoxins in seawater using a novel molecularly imprinted nanoprobe
	Xu, Huan 1; Lian, Ziru 1; Hao, Xiaochen 1; Li, Fang 1; Yu, Ren-Cheng 2,3
	2024-02-20
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	912 页码:11
通讯作者	Lian, Ziru(yoyo-lzr@hotmail.com)
摘要	Gonyautoxins (GTXs), a group of potent neurotoxins belonging to paralytic shellfish toxins (PSTs), are often associated with harmful algal blooms of toxic dinoflagellates in the sea and represent serious health and ecological concerns worldwide. In the study, a highly selective and sensitive fluorescence nanoprobe was con-structed based on photoinduced electron transfer recognition mechanism to rapidly detect GTXs in seawater, using specific entrapment of molecularly imprinted polymers (MIPs) combined with fluorescence analyses. The green emissive fluorescein isothiocyanate was grafted in a silicate matrix as a signal transducer and fluorescence intensity of the nanoprobe with a core-shell structure exhibited a strong enhancement due to efficient analyte blockage in a short response time. Under optimal conditions, the developed MIPs nanoprobe presented an excellent analytical performance for spiked seawater samples including a recovery from 94.44 % to 98.23 %, a linear range between 0.018 nmol L-1 and 0.36 nmol L-1, as well as good accuracy. Furthermore, the method had extremely high sensitivity, with limit of detection obtained as 0.005 nmol L-1 for GTXs and GTX2/3. Finally, the nanoprobe was applied for the determination of GTXs in seven natural seawater samples with GTXs mixture (0.035-0.058 nmol L-1) or single GTX2/3 (0.033-0.050 nmol L-1), and the results agreed well with those of a UPLC-MS/MS method. The findings of our study suggest that the constructed MIPs-based fluorescence enhancement nanoprobe was suitable for rapid, selective and ultrasensitive detection of GTXs, particular GTX2/ 3, in natural seawater samples.
关键词	Gonyautoxins Fluorescence enhancement nanoprobe Seawater Ultrasensitive detection Surface molecular imprinting
DOI	10.1016/j.scitotenv.2023.169056
收录类别	SCI
语种	英语
资助项目	Joint Funds of the National Natural Science Foundation of China[U22A20583] ; Taishan Scholars Program
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:001133920800001
出版者	ELSEVIER
WOS关键词	PARALYTIC SHELLFISH TOXINS ; SOLID-PHASE EXTRACTION ; LIQUID-CHROMATOGRAPHY ; POLYMER ; OXIDATION ; BLOOMS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184228
专题	海洋生态与环境科学重点实验室
通讯作者	Lian, Ziru
作者单位	1.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Huan,Lian, Ziru,Hao, Xiaochen,et al. Ultrasensitive fluorescence detection of gonyautoxins in seawater using a novel molecularly imprinted nanoprobe[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2024,912:11.
APA	Xu, Huan,Lian, Ziru,Hao, Xiaochen,Li, Fang,&Yu, Ren-Cheng.(2024).Ultrasensitive fluorescence detection of gonyautoxins in seawater using a novel molecularly imprinted nanoprobe.SCIENCE OF THE TOTAL ENVIRONMENT,912,11.
MLA	Xu, Huan,et al."Ultrasensitive fluorescence detection of gonyautoxins in seawater using a novel molecularly imprinted nanoprobe".SCIENCE OF THE TOTAL ENVIRONMENT 912(2024):11.