IOCAS-IR  > 海洋生态与环境科学重点实验室
Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China
He, Ziyan1,2,3,4; Liu, Shuya1,2,4; Cui, Zongmei1,2,4; Zhao, Yongfang2,4,5; Chen, Nansheng1,2,4,6
2023-11-13
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Pages14
Corresponding AuthorChen, Nansheng(chenn@qdio.ac.cn)
AbstractPseudo-nitzschia is a species-rich genus with many species able to induce harmful algae blooms (HABs) associated with the toxin, domoic acid (DA), production. Despite their high diversity revealed by taxonomical studies, the composition and spatial-temporal dynamics of Pseudo-nitzschia species in marine ecological systems are often inadequately resolved due to the insufficient resolution associated with morphology-based approaches applied in ecological studies. In this study, metabarcoding analysis was applied to identify Pseudo-nitzschia species and track their spatial-temporal dynamics in Jiaozhou Bay (JZB), a model marine ecosystem located in Qingdao, China. This metabarcoding analysis potentially uncovered 18 Pseudo-nitzschia species, including three Pseudo-nitzschia species identified in JZB in previous microscopy-based studies, four Pseudo-nitzschia species (P. micropora, P. multistriata, P. galaxiae, and P. fraudulenta) that have not been identified in JZB, and 11 potential Pseudo-nitzschia species whose molecular marker sequences are identical, demonstrating the strength of metabarcoding analysis in ecological research. Furthermore, this metabarcoding analysis also revealed differential spatial-temporal dynamics of many Pseudo-nitzschia species. While P. galaxiae was found to show a strong preference for summer months, P. pungens showed relatively high abundance in later summer and early autumn, P. delicatissima and P. multistriata showed higher abundance in autumn and winter, and P. micropora was more common in July. To ascertain driving forces for such differential seasonal preferences, we evaluated the impact of environmental factors on the spatial-temporal preferences of the Pseudo-nitzschia species, which revealed that temperature and other factors together contributed to the differential dynamic changes. This study demonstrated that metabarcoding analysis is an effective approach for tracking the composition and spatial-temporal dynamics of Pseudo-nitzschia species in marine ecological systems.
KeywordPseudo-nitzschia Metabarcoding analysis Jiaozhou Bay Harmful algal bloom Spatial-temporal dynamics
DOI10.1007/s10811-023-03127-4
Indexed BySCI
Language英语
Funding ProjectWe are grateful to colleagues from the Jiaozhou Bay Marine Ecosystem Research Station for the opportunity to participate in the investigation expeditions. Data analysis was supported by Oceanographic Data Center, IOCAS.
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:001102562000004
PublisherSPRINGER
WOS KeywordNORTHWESTERN ADRIATIC SEA ; DOMOIC ACID ; ENVIRONMENTAL DNA ; PHYTOPLANKTON ; BACILLARIOPHYCEAE ; BIODIVERSITY ; SEASONALITY ; PHYLOGENY ; DIVERSITY ; COMMUNITY
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/184225
Collection海洋生态与环境科学重点实验室
胶州湾海洋生态系统国家野外研究站
Corresponding AuthorChen, Nansheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 10039, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China
6.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
He, Ziyan,Liu, Shuya,Cui, Zongmei,et al. Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China[J]. JOURNAL OF APPLIED PHYCOLOGY,2023:14.
APA He, Ziyan,Liu, Shuya,Cui, Zongmei,Zhao, Yongfang,&Chen, Nansheng.(2023).Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China.JOURNAL OF APPLIED PHYCOLOGY,14.
MLA He, Ziyan,et al."Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China".JOURNAL OF APPLIED PHYCOLOGY (2023):14.
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