Metabarcoding analysis of the composition and spatial-temporal dynamics of <i>Pseudo-nitzschia</i> species in Jiaozhou Bay, China

doi:10.1007/s10811-023-03127-4

	*Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia* species in Jiaozhou Bay, China**
	He, Ziyan 1,2,3,4; Liu, Shuya 1,2,4; Cui, Zongmei 1,2,4; Zhao, Yongfang 2,4,5; Chen, Nansheng 1,2,4,6
	2023-11-13
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
页码	14
通讯作者	Chen, Nansheng(chenn@qdio.ac.cn)
摘要	Pseudo-nitzschia is a species-rich genus with many species able to induce harmful algae blooms (HABs) associated with the toxin, domoic acid (DA), production. Despite their high diversity revealed by taxonomical studies, the composition and spatial-temporal dynamics of Pseudo-nitzschia species in marine ecological systems are often inadequately resolved due to the insufficient resolution associated with morphology-based approaches applied in ecological studies. In this study, metabarcoding analysis was applied to identify Pseudo-nitzschia species and track their spatial-temporal dynamics in Jiaozhou Bay (JZB), a model marine ecosystem located in Qingdao, China. This metabarcoding analysis potentially uncovered 18 Pseudo-nitzschia species, including three Pseudo-nitzschia species identified in JZB in previous microscopy-based studies, four Pseudo-nitzschia species (P. micropora, P. multistriata, P. galaxiae, and P. fraudulenta) that have not been identified in JZB, and 11 potential Pseudo-nitzschia species whose molecular marker sequences are identical, demonstrating the strength of metabarcoding analysis in ecological research. Furthermore, this metabarcoding analysis also revealed differential spatial-temporal dynamics of many Pseudo-nitzschia species. While P. galaxiae was found to show a strong preference for summer months, P. pungens showed relatively high abundance in later summer and early autumn, P. delicatissima and P. multistriata showed higher abundance in autumn and winter, and P. micropora was more common in July. To ascertain driving forces for such differential seasonal preferences, we evaluated the impact of environmental factors on the spatial-temporal preferences of the Pseudo-nitzschia species, which revealed that temperature and other factors together contributed to the differential dynamic changes. This study demonstrated that metabarcoding analysis is an effective approach for tracking the composition and spatial-temporal dynamics of Pseudo-nitzschia species in marine ecological systems.
关键词	Pseudo-nitzschia Metabarcoding analysis Jiaozhou Bay Harmful algal bloom Spatial-temporal dynamics
DOI	10.1007/s10811-023-03127-4
收录类别	SCI
语种	英语
资助项目	We are grateful to colleagues from the Jiaozhou Bay Marine Ecosystem Research Station for the opportunity to participate in the investigation expeditions. Data analysis was supported by Oceanographic Data Center, IOCAS.
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:001102562000004
出版者	SPRINGER
WOS关键词	NORTHWESTERN ADRIATIC SEA ; DOMOIC ACID ; ENVIRONMENTAL DNA ; PHYTOPLANKTON ; BACILLARIOPHYCEAE ; BIODIVERSITY ; SEASONALITY ; PHYLOGENY ; DIVERSITY ; COMMUNITY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184225
专题	海洋生态与环境科学重点实验室胶州湾海洋生态系统国家野外研究站
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 10039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China 6.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	He, Ziyan,Liu, Shuya,Cui, Zongmei,et al. Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China[J]. JOURNAL OF APPLIED PHYCOLOGY,2023:14.
APA	He, Ziyan,Liu, Shuya,Cui, Zongmei,Zhao, Yongfang,&Chen, Nansheng.(2023).Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China.JOURNAL OF APPLIED PHYCOLOGY,14.
MLA	He, Ziyan,et al."Metabarcoding analysis of the composition and spatial-temporal dynamics of Pseudo-nitzschia species in Jiaozhou Bay, China".JOURNAL OF APPLIED PHYCOLOGY (2023):14.

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