The evolutionary diversification and antimicrobial potential of MPEG1 in Metazoa

doi:10.1016/j.csbj.2023.11.032

IOCAS-IR > 实验海洋生物学重点实验室

	The evolutionary diversification and antimicrobial potential of MPEG1 in Metazoa
	Chen, Yuan1,2,3 ; Yuan, Zihao1,2,3 ; Sun, Li 1,2,3
	2023
发表期刊	COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
ISSN	2001-0370
卷号	21 页码:5818-5828
通讯作者	Yuan, Zihao(yuanzihao@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
摘要	Macrophage-expressed gene 1 (MPEG1) is an ancient immune effector known to exist in Cnidaria, Mollusca, Actinopterygii, and Mammalia. In this study, we examined the evolution and antibacterial potential of MPEG1 across Metazoa. By unbiased data-mining, MPEG1 orthologs were found in 11 of 34 screened phyla. In invertebrates, MPEG1 is present in the major phyla and exhibits intensive duplication. In vertebrates, class-based clades were formed by the major, generic MPEG1 (gMPEG1) in each class. However, there is a minority of unique MPEG1 (uMPEG1) from 71 species of 4 classes that clustered into a separate clade detached from all major classbased clades. gMPEG1 and uMPEG1 exhibit strong genomic collinearity and are surrounded by high-density transposons. gMPEG1 and uMPEG1 transcript expressions were most abundant in immune organs, but differed markedly in tissue specificity. Systematic analysis identified an antimicrobial peptide (AMP)-like segment in the C-terminal (CT) tail of MPEG1. Peptides based on the AMP-like regions of 35 representative MPEG1 were synthesized. Bactericidal activities were displayed by all peptides. Together these results suggest transposonpropelled evolutionary diversification of MPEG1 in Metazoa that has likely led to functional specialisation. This study also reveals a possible antimicrobial mechanism mediated directly and solely by the CT tail of MPEG1.
关键词	Macrophage-expressed gene 1/Perforin 2 Data mining Evolution Metazoa Antimicrobial
DOI	10.1016/j.csbj.2023.11.032
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050402] ; Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] ; Innovation Research Group Project of the National Natural Science Foundation of China[42221005]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:001126174400001
出版者	ELSEVIER
WOS关键词	DE-NOVO IDENTIFICATION ; PERFORIN-2 ; EXPRESSION ; ABALONE ; FAMILIES ; GENES
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184186
专题	实验海洋生物学重点实验室
通讯作者	Yuan, Zihao; Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen, Yuan,Yuan, Zihao,Sun, Li. The evolutionary diversification and antimicrobial potential of MPEG1 in Metazoa[J]. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,2023,21:5818-5828.
APA	Chen, Yuan,Yuan, Zihao,&Sun, Li.(2023).The evolutionary diversification and antimicrobial potential of MPEG1 in Metazoa.COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,21,5818-5828.
MLA	Chen, Yuan,et al."The evolutionary diversification and antimicrobial potential of MPEG1 in Metazoa".COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 21(2023):5818-5828.