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Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer
Song, Wen1,2,3,4; Xing, Ronge1,2,3; Yang, Haoyue1,2; Gao, Kun1,2; Liu, Song1,2,3; Li, Xin1,2; Yu, Huahua1,2,3; Li, Pengcheng1,2,3
2023-10-01
Source PublicationLWT-FOOD SCIENCE AND TECHNOLOGY
ISSN0023-6438
Volume188Pages:13
Corresponding AuthorXing, Ronge(xingronge@qdio.ac.cn)
AbstractThe investigation into natural eumelanin found in squid ink is an emerging research topic due to its antioxidative properties. However, the methodologies for acquiring pure eumelanin and establishing the intricate relationship between its structure and antioxidative efficacy remain limited. Novel enzyme extraction technique, termed response surface optimization, was employed to synthesize high-purity eumelanin, and its antioxidant activity was evaluated at free radical scavenging and cellular levels and correlated with detailed tetrameric structure prediction for the first time. Results demonstrated that enzymatic extraction technique yielded eumelanin (ANF) nanostructure with stability, boasting particle size of 112.35 nm, a maximum absorption wavelength of 224 nm, a Delta Hpp value of 0.4802 mT, and a purity of 92%. Compared with melanin standard (MelS), ANF had superior free radical scavenging activity, eliminating 89.62% of DPPH radicals, 96.79% of superoxide ion radicals, and 93.20% of hydroxyl radicals, and better repair of cellular oxidative damage. Based on molecular energy, molecular electrostatic potential (MEP), and molecular orbitals by Gaussian calculations, the tetrameric structure (DHICA3-DHI1"' and "DHICA3-DHI1") with significant antioxidant activity contained inside eumelanin was anticipated. Our findings reveal its potential as a natural antioxidant, offering broad prospects for antioxidant food additives or therapeutic applications in nutritional and pharmaceutical ingredients.
KeywordEumelanin Enzyme -assisted optimization Squid ink Tetramer Antioxidant activity
DOI10.1016/j.lwt.2023.115464
Indexed BySCI
Language英语
Funding ProjectShandong Key R D Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Fujian Science and Technology Planning Project -STS Program[2021T3013]
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:001109520500001
PublisherELSEVIER
WOS KeywordMELANIN ; PROTEINS ; BEHAVIOR ; PIGMENT ; VIVO
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/183955
Collection深海极端环境与生命过程研究中心
Corresponding AuthorXing, Ronge
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 7 Nanhai Rd, Qingdao 266000, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100000, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song, Wen,Xing, Ronge,Yang, Haoyue,et al. Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer[J]. LWT-FOOD SCIENCE AND TECHNOLOGY,2023,188:13.
APA Song, Wen.,Xing, Ronge.,Yang, Haoyue.,Gao, Kun.,Liu, Song.,...&Li, Pengcheng.(2023).Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer.LWT-FOOD SCIENCE AND TECHNOLOGY,188,13.
MLA Song, Wen,et al."Eumelanin: A natural antioxidant isolated from squid ink by new enzymatic technique and prediction of its structural tetramer".LWT-FOOD SCIENCE AND TECHNOLOGY 188(2023):13.
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