Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas)

doi:10.1007/s42995-023-00204-y

IOCAS-IR > 实验海洋生物学重点实验室

	Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas)
	Xu, Fei 1,2,3,4; Deng, Shaoxi1,2,5,6 ; Gavriouchkina, Daria 7,8; Zhang, Guofan 1,2,3,4
	2023-11-25
发表期刊	MARINE LIFE SCIENCE & TECHNOLOGY
ISSN	2096-6490
页码	11
通讯作者	Xu, Fei(xufei@qdio.ac.cn)
摘要	Many marine invertebrate phyla are characterized by indirect development. These animals transit from planktonic larvae to benthic spats via settlement and metamorphosis, which contributes to their adaption to the marine environment. Studying the biological process of metamorphosis is, thus, key to understanding the origin and evolution of indirect development. Although numerous studies have been conducted on the relationship between metamorphosis and the marine environment, microorganisms, and neurohormones, little is known about gene regulation network (GRN) dynamics during metamorphosis. Metamorphosis-competent pediveligers of the Pacific oyster Crassostrea gigas were assayed in this study. By assaying gene expression patterns and open chromatin region changes of different samples of larvae and spats, the dynamics of molecular regulation during metamorphosis were examined. The results indicated significantly different gene regulation networks before, during and post-metamorphosis. Genes encoding membrane-integrated receptors and those related to the remodeling of the nervous system were upregulated before the initiation of metamorphosis. Massive biogenesis, e.g., of various enzymes and structural proteins, occurred during metamorphosis as inferred from the comprehensive upregulation of the protein synthesis system post epinephrine stimulation. Hierarchical downstream gene networks were then stimulated. Some transcription factors, including homeobox, basic helix-loop-helix and nuclear receptors, showed different temporal response patterns, suggesting a complex GRN during the transition stage. Nuclear receptors, as well as their retinoid X receptor partner, may participate in the GRN controlling oyster metamorphosis, indicating an ancient role of the nuclear receptor regulation system in animal metamorphosis.
关键词	Gene regulation network Indirect development Lophotrochozoa Nuclear receptors Neurohormones Protein synthesis Transcription factors
DOI	10.1007/s42995-023-00204-y
收录类别	SCI
语种	英语
资助项目	Qingdao Frontier Ocean Seed Company Ltd ; Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203001] ; Key Research and Development Program of Shandong[2022LZGC015] ; Taishan Scholars Program
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:001107306100001
出版者	SPRINGERNATURE
WOS关键词	BODY PLANS ; GENES ; ORIGINS ; MECHANISMS ; EXPRESSION ; PHYLOGENY ; EVOLUTION ; ARAGONITE ; ALIGNMENT ; MOLLUSKS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183937
专题	实验海洋生物学重点实验室
通讯作者	Xu, Fei
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Laoshan Lab, Qingdao 266237, Peoples R China 4.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao 266105, Peoples R China 5.City Univ Hong Kong, Sch Energy & Environm, Hong Kong 999077, Peoples R China 6.City Univ Hong Kong, State Key Lab Marine Pollut, Hong Kong 999077, Peoples R China 7.Okinawa Inst Sci & Technol Grad Univ, Mol Genet Unit, Onna, Okinawa 9040495, Japan 8.UCL, UK Dementia Res Inst, London WC1E 6BT, England
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xu, Fei,Deng, Shaoxi,Gavriouchkina, Daria,et al. Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas)[J]. MARINE LIFE SCIENCE & TECHNOLOGY,2023:11.
APA	Xu, Fei,Deng, Shaoxi,Gavriouchkina, Daria,&Zhang, Guofan.(2023).Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas).MARINE LIFE SCIENCE & TECHNOLOGY,11.
MLA	Xu, Fei,et al."Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas)".MARINE LIFE SCIENCE & TECHNOLOGY (2023):11.