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ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress
Zhou, Cong1,2,3,4,5,6; Zou, Yan7; Hu, Zhi1,2,3,4,5,6; Yang, Mei-jie1,2,3,4,6; Shi, Pu1,2,3,4,5,6; Li, Yong-ren8; Guo, Yong-jun8; Zhang, Tao1,2,3,4,6,9; Song, Hao1,2,3,4,6,9
2023-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume141Pages:11
Corresponding AuthorZhang, Tao(tzhang@qdio.ac.cn) ; Song, Hao(haosong@qdio.ac.cn)
AbstractAir exposure (AE) is a significant environmental stressor that can lead to desiccation, hypoxia, starvation, and disruption of cellular homeostasis in marine bivalves. Autophagy is a highly conserved catabolic pathway that facilitates the degradation of damaged macromolecules and organelles, thereby supporting cellular stress responses. To date, autophagy-mediated resistance mechanisms to AE stress remain largely elusive in bivalves. In this study, we performed a multi-tool approach to investigate the autophagy-related physiological regulation in hard clams (Mercenaria mercenaria) under different duration of AE (T = 0, 1, 5, 10, 20, 30 days). We observed that autophagy of haemocytes was significantly activated on day 5. However, autophagy activity began to significantly decline from day 10 to day 30. Autophagy was significantly inhibited after antioxidant treatment, indicating that reactive oxygen species (ROS) was an endogenous inducer of autophagy. A significant decline in the survival rate of hard clams was observed after injection of ammonium chloride or carbamazepine during AE stress, suggesting that moderate autophagy was conducive for clam survival under AE stress. We also observed DNA breaks and high levels of apoptosis in haemocytes on day 10. Activation of apoptosis lagged behind autophagy, and the relationship between autophagy and apoptosis might shift from antagonism to synergy with the duration of stress. This study provides novel insights into the stress resistance mechanisms in marine bivalves.
KeywordAir exposure Resistance Haemocytes Oxidative stress Autophagy
DOI10.1016/j.fsi.2023.109084
Indexed BySCI
Language英语
Funding ProjectCreative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[2021AB34014] ; Young Elite Scientists Sponsorship Program by CAST[LMEES-CTSP-2018-1] ; Youth Innovation Promotion Association CAS[2021QNRC001]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:001101017000001
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS KeywordOXIDATIVE STRESS ; MYTILUS-GALLOPROVINCIALIS ; LYSOSOMAL REACTIONS ; CELL-DEATH ; APOPTOSIS ; RESPONSES ; IDENTIFICATION ; TEMPERATURE ; MECHANISMS ; PARAMETERS
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/183895
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhang, Tao; Song, Hao
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
7.Marine Sci Res Inst Shandong Prov, Qingdao 266100, Peoples R China
8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin 300384, Peoples R China
9.7 Nanhai Rd, Qingdao City, Shandong, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Cong,Zou, Yan,Hu, Zhi,et al. ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress[J]. FISH & SHELLFISH IMMUNOLOGY,2023,141:11.
APA Zhou, Cong.,Zou, Yan.,Hu, Zhi.,Yang, Mei-jie.,Shi, Pu.,...&Song, Hao.(2023).ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress.FISH & SHELLFISH IMMUNOLOGY,141,11.
MLA Zhou, Cong,et al."ROS-induced moderate autophagy of haemocytes confers resistance of Mercenaria mercenaria to air exposure stress".FISH & SHELLFISH IMMUNOLOGY 141(2023):11.
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