Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture

doi:10.1016/j.theriogenology.2023.09.013

IOCAS-IR > 实验海洋生物学重点实验室

	Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture
	Duan, Lei 1,2,3; Du, Shuran 1,2; Wang, Xueying1,2 ; Zhou, Li 1,2; Liu, Qinghua 1,2; Li, Jun 1,2
	2024-01-15
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	214 页码:1-9
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	Germ cell transplantation (GCT) is a promising biotechnology that enables the production of donor-derived gametes in surrogate recipients. It plays a crucial role in the protection of endangered species, the propaga-tion of elite species with desired traits, and long-term preservation of genetic resources. This significance is particularly pronounced when GCT is synergistically employed with cryopreservation techniques. However, GCT often encounters challenges due to low colonization rates and, in some cases, complete loss of donor cells in recipients. Glial cell line-derived neurotrophic factor (GDNF) plays a pivotal role in sustaining the self-renewal of spermatogonial stem cells (SSCs) in mammals. Additionally, it has been shown to promote the proliferation of spermatogonia in vitro cultures in certain animal species. In turbot (Scophthalmus maximus), we found that the expressions of gdnf and gfr alpha 1a were predominantly observed in spermatogonia rather than somatic cells, which differed from their expression patterns in mammals. The efficiency of exogenous spermatogonia transplantation in Japanese flounder (Paralichthys olivaceus) larvae could be substantially enhanced by incubating donor cells from turbot with 100 ng/ml GDNF prior to transplantation. This led to a noteworthy increase in the colonization rate, rising from 33%-50%-61.5%. Additionally, the addition of 20 ng/ml GDNF in cell medium could also promote the proliferation of turbot germ cells in vitro. These results demonstrated the gdnf in turbot testis expression characteristics and suggested that addition of GNDF could be an effective way to improve the GCT efficiency and promote the germ cells expansion during in vitro culture.
关键词	GDNF Germ cells Germ cell transplantation Turbot
DOI	10.1016/j.theriogenology.2023.09.013
收录类别	SCI
语种	英语
资助项目	Shandong Province Natural Science Foundation[ZR2020KC038] ; Research and Development Program of Shandong Province[2021LZGC029] ; China Agriculture Research System[CARS-47]
WOS研究方向	Reproductive Biology ; Veterinary Sciences
WOS类目	Reproductive Biology ; Veterinary Sciences
WOS记录号	WOS:001098779600001
出版者	ELSEVIER SCIENCE INC
WOS关键词	SPERMATOGONIAL STEM-CELLS ; YELLOWTAIL SERIOLA-QUINQUERADIATA ; RAINBOW-TROUT ; PROLIFERATION ; TRANSPLANTATION ; MAINTENANCE ; GENERATION ; SURROGATE ; SURVIVAL ; TESTIS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183850
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Duan, Lei,Du, Shuran,Wang, Xueying,et al. Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture[J]. THERIOGENOLOGY,2024,214:1-9.
APA	Duan, Lei,Du, Shuran,Wang, Xueying,Zhou, Li,Liu, Qinghua,&Li, Jun.(2024).Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture.THERIOGENOLOGY,214,1-9.
MLA	Duan, Lei,et al."Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture".THERIOGENOLOGY 214(2024):1-9.