Molecular imprinting-based indirect fluorescence detection strategy implemented on paper chip for non-fluorescent microcystin

doi:10.1038/s41467-023-42244-z

	Molecular imprinting-based indirect fluorescence detection strategy implemented on paper chip for non-fluorescent microcystin
	Li, Bowei 1,2; Qi, Ji 1,2; Liu, Feng 1; Zhao, Rongfang 1,2; Arabi, Maryam 1,2; Ostovan, Abbas 1,2; Song, Jinming2,3,4 ; Wang, Xiaoyan 5; Zhang, Zhiyang 1,2; Chen, Lingxin 1,2,6
	2023-10-17
发表期刊	NATURE COMMUNICATIONS
卷号	14 期号:1 页码:12
通讯作者	Qi, Ji(jqi@yic.ac.cn) ; Song, Jinming(jmsong@qdio.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
摘要	Fluorescence analysis is a fast and sensitive method, and has great potential application in trace detection of environmental toxins. However, many important environmental toxins are non-fluorescent substances, and it is still a challenge to construct a fluorescence detection method for non-fluorescent substances. Here, by means of charge transfer effect and smart molecular imprinting technology, we report a sensitive indirect fluorescent sensing mechanism (IFSM) and microcystin (MC-RR) is selected as a model target. A molecular imprinted thin film is immobilized on the surface of zinc ferrite nanoparticles (ZnFe2O4 NPs) by using arginine, a dummy fragment of MC-RR. By implementation of IFSM on the paper-based microfluidic chip, a versatile platform for the quantitative assay of MC-RR is developed at trace level (the limit of detection of 0.43 mu g/L and time of 20 min) in real water samples without any pretreatment. Importantly, the proposed IFSM can be easily modified and extended for the wide variety of species which lack direct interaction with the fluorescent substrate. This work offers the potential possibility to meet the requirements for the on-site analysis and may explore potential applications of molecularly imprinted fluorescent sensors.
DOI	10.1038/s41467-023-42244-z
收录类别	SCI
语种	英语
资助项目	This work was financially supported by the National Natural Science Foundation of China (Grant No. 22106179 to J.Q., 42376188 to B.L., 22376216 to L.C., 41776110 to B.L. and 22374162 to M.A.), Shandong Provincial Natural Science Foundation Key Project (ZR2[22106179] ; This work was financially supported by the National Natural Science Foundation of China (Grant No. 22106179 to J.Q., 42376188 to B.L., 22376216 to L.C., 41776110 to B.L. and 22374162 to M.A.), Shandong Provincial Natural Science Foundation Key Project (ZR2[42376188] ; This work was financially supported by the National Natural Science Foundation of China (Grant No. 22106179 to J.Q., 42376188 to B.L., 22376216 to L.C., 41776110 to B.L. and 22374162 to M.A.), Shandong Provincial Natural Science Foundation Key Project (ZR2[22376216] ; This work was financially supported by the National Natural Science Foundation of China (Grant No. 22106179 to J.Q., 42376188 to B.L., 22376216 to L.C., 41776110 to B.L. and 22374162 to M.A.), Shandong Provincial Natural Science Foundation Key Project (ZR2[41776110] ; This work was financially supported by the National Natural Science Foundation of China (Grant No. 22106179 to J.Q., 42376188 to B.L., 22376216 to L.C., 41776110 to B.L. and 22374162 to M.A.), Shandong Provincial Natural Science Foundation Key Project (ZR2[22374162] ; National Natural Science Foundation of China[ZR2020KB022] ; National Natural Science Foundation of China[ZR2023MB003] ; Shandong Provincial Natural Science Foundation Key Project[LQ-GJ-03-02] ; Key Technology Projects of Equipment for Cold-seep Ecosystem Research[2019PC0050] ; Key Technology Projects of Equipment for Cold-seep Ecosystem Research[2020PC0083] ; Chinese Academy of Sciences President's International Fellowship Initiative[ts20190962] ; Taishan Scholar Project Special Funding
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001086900900010
出版者	NATURE PORTFOLIO
WOS关键词	POLYMER NANOPARTICLES ; MICROFLUIDIC CHIP ; LR ; IMMUNOASSAY ; APTASENSOR ; DOTS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183829
专题	海洋生态与环境科学重点实验室
通讯作者	Qi, Ji; Song, Jinming; Chen, Lingxin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 6.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Li, Bowei,Qi, Ji,Liu, Feng,et al. Molecular imprinting-based indirect fluorescence detection strategy implemented on paper chip for non-fluorescent microcystin[J]. NATURE COMMUNICATIONS,2023,14(1):12.
APA	Li, Bowei.,Qi, Ji.,Liu, Feng.,Zhao, Rongfang.,Arabi, Maryam.,...&Chen, Lingxin.(2023).Molecular imprinting-based indirect fluorescence detection strategy implemented on paper chip for non-fluorescent microcystin.NATURE COMMUNICATIONS,14(1),12.
MLA	Li, Bowei,et al."Molecular imprinting-based indirect fluorescence detection strategy implemented on paper chip for non-fluorescent microcystin".NATURE COMMUNICATIONS 14.1(2023):12.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
s41467-023-42244-z.p（2790KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览