Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria

doi:10.1016/j.csbj.2022.08.004

	Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria
	Zhou, Cong1,2,3,4,5,6 ; Song, Hao 1,2,3,4,6; Feng, Jie 1,2,3,4; Hu, Zhi 1,2,3,4,5; Yang, Mei-jie 1,2,3,4,5; Shi, Pu1,2,3,4,5 ; Li, Yong-ren 7; Guo, Yong-jun 7; Li, Hai-zhou 8; Zhang, Tao1,2,3,4,6
	2022
发表期刊	COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
ISSN	2001-0370
卷号	20 页码:4110-4121
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn)
摘要	Hypo-salinity events frequently occur in marine ecosystem due to persistent rainfall and freshwater inflow, reducing the cytosol osmolarity and triggering cellular stress responses in aquatic organisms. Euryhaline bivalves have developed sophisticated regulatory mechanisms to adapt to salinity fluctuations over a long period of evolution. In this study, we performed multiple biochemical assays, widely targeted metabolomics, and gene expression analysis to investigate the comprehensive metabolic responses to hypo-salinity stress and osmoregulation mechanisms in hard clam Mercenaria mercenaria, which is a euryhaline bivalve species widely cultured in China. During hypo-salinity stress, increased vacuoles appeared in gill filaments. The Na+ and Cl- concentrations in gills significantly decreased because of the up-regulation of Na+/K+-ATPase (NKA) activity. The cAMP content dramatically decreased at 5 d post hypo-salinity stress. Meanwhile, the gene expression levels of adenylate cyclase, proteinkinase A, and sodium and calcium channel proteins were evidently down-regulated, suggesting that cAMP-PKA pathway was inhibited to prevent ambient inorganic ions from entering the gill cells. Antioxidant metabolites, such as serine and Tyr-containing dipeptides, were significantly up-regulated to resist oxidative stress. Glycerolipid metabolism was strengthened to stabilize membrane structure when hypo-salinity stress was prolonged to 5 days. At 1 d post hypo-salinity stress, an increase in alanine and lactate contents marked the initiation of anaerobic metabolism. Acylcarnitines accumulation indicated that fatty acids b-oxidation was promoted to provide energy for osmoregulation. The potential biomarkers of hyposalinity stress were identified in hard clams. This study provides novel insights into the metabolic regulatory mechanisms to hypo-salinity stress in euryhaline bivalves. (c) 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词	Metabolomics Osmoregulation Oxidative stress Energy metabolism Hypo -salinity tolerance
DOI	10.1016/j.csbj.2022.08.004
收录类别	SCI
语种	英语
资助项目	China National Key R D Program[2019YFD0900800] ; Tianjin science and technology commission project[20YFZCSN00240] ; Tianjin Agricultural Committee Project[202103010] ; China Agriculture Research System of MOF and MARA ; Taishan Industrial Leading Talents Project[LJNY201704] ; Double Hundred Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; Young Elite Scientists Sponsorship Program by CAST[2021QNRC001] ; Shandong Province Agricultural Major Applied Technology Innovation Project[SF1405303301]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000919216100008
出版者	ELSEVIER
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183413
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300384, Peoples R China 8.Shandong Fu Han Ocean Sci Tech Co Ltd, Haiyang 265100, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Cong,Song, Hao,Feng, Jie,et al. Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria[J]. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,2022,20:4110-4121.
APA	Zhou, Cong.,Song, Hao.,Feng, Jie.,Hu, Zhi.,Yang, Mei-jie.,...&Zhang, Tao.(2022).Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria.COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,20,4110-4121.
MLA	Zhou, Cong,et al."Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria".COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 20(2022):4110-4121.