Evaluating the Utility of Five Gene Fragments for Genetic Diversity Analyses of Mytella strigata Populations

doi:10.3390/fishes8010034

	Evaluating the Utility of Five Gene Fragments for Genetic Diversity Analyses of Mytella strigata Populations
	Zuo, Chenxia 1,2; Zhang, Tingting 2; Zhang, Chenchen 2; Zhao, Daotan 2; Zhu, Yi 1,3; Ma, Xiaojie 1,3; Wang, Haiyan 1; Ma, Peizhen1 ; Zhang, Zhen 1,3
	2023
发表期刊	FISHES
卷号	8 期号:1 页码:10
通讯作者	Ma, Peizhen(mapeizhen@qdio.ac.cn) ; Zhang, Zhen(zhangzhen@qdio.ac.cn)
摘要	Mytella strigata (Hanley, 1843) is an invasive mussel species that has rapidly spread in China in recent years. Here, we tested the utility of three mitochondrial gene fragments, COI, 12S, and 16S, and two nuclear gene fragments, D1 28S and 18S-ITS1, for characterizing the levels of genetic diversity among and within populations using 191 M. strigata specimens collected in China to aid ongoing efforts to identify the origin of the invasion as well as molecular genetic studies. M. strigata exhibited two sex-associated haplogroups according to the COI and 12S sequences. The ratio of female-lineage to male-lineage COI and 12S sequences was 149:22 and 72:7, and the genetic distances between haplogroups were 6.56 and 9.17, respectively. Only one haplotype was detected among the 18S-ITS1 sequences (413 bp), and three haplotypes were detected among the D1 28S sequences (296 bp). The haplotype diversity of both the female-lineage COI and 12S sequences was greater than 0.5, and the nucleotide diversity of the 12S, 16S, D1 28S, and 18S-ITS1 sequences was less than 0.005 in all six populations in China. Our findings indicated that COI is the most useful gene fragment for genetic diversity studies of M. strigata populations; D1 28S and 18S-ITS1 sequences would be useful for species identification because of their low intraspecific diversity. Our genetic analysis of the COI sequences revealed Colombia as the most likely origin of M. strigata in China and showed that the invasive populations in China have recently experienced or are currently experiencing a population bottleneck.
关键词	adaptive evolution mitochondrial cytochrome oxidase subunit I biological invasion mussel molecular marker
DOI	10.3390/fishes8010034
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000914966200001
出版者	MDPI
WOS关键词	DOUBLY UNIPARENTAL INHERITANCE ; 2 MITOCHONDRIAL GENOMES ; BIVALVIA MYTILIDAE ; 1ST RECORD ; HANLEY ; DNA ; EVOLUTION ; IMPACTS ; DUI
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183386
专题	海洋生物分类与系统演化实验室
通讯作者	Ma, Peizhen; Zhang, Zhen
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zuo, Chenxia,Zhang, Tingting,Zhang, Chenchen,et al. Evaluating the Utility of Five Gene Fragments for Genetic Diversity Analyses of Mytella strigata Populations[J]. FISHES,2023,8(1):10.
APA	Zuo, Chenxia.,Zhang, Tingting.,Zhang, Chenchen.,Zhao, Daotan.,Zhu, Yi.,...&Zhang, Zhen.(2023).Evaluating the Utility of Five Gene Fragments for Genetic Diversity Analyses of Mytella strigata Populations.FISHES,8(1),10.
MLA	Zuo, Chenxia,et al."Evaluating the Utility of Five Gene Fragments for Genetic Diversity Analyses of Mytella strigata Populations".FISHES 8.1(2023):10.