A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein

doi:10.3390/v15010227

IOCAS-IR > 实验海洋生物学重点实验室

	A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein
	Sun, Mingzhe1 ; Li, Shihao 1,2,3; Yu, Yang1,2,3 ; Zhang, Xiaojun 1,2,3; Li, Fuhua 1,2,3,4
	2023
发表期刊	VIRUSES-BASEL
卷号	15 期号:1 页码:16
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
摘要	Hemocytes are essential components of the immune system against invading pathogens in shrimp. Many uncharacterized transcripts exist in hemocytes but the knowledge of them is very limited. In the present study, we identified a novel small protein from the uncharacterized transcripts in hemocytes of Litopenaeus vannamei. This transcript was specifically expressed in hemocytes and encoded a novel secretory protein, which was designated as hemocyte-specific small protein (LvHSSP). The expression level of LvHSSP was significantly up-regulated in the hemocytes of shrimp infected with white spot syndrome virus (WSSV). After knockdown of LvHSSP by RNA interference, the WSSV copy number in shrimp decreased significantly. Conversely, WSSV copy number increased in shrimp when they were infected by WSSV after incubation with recombinant LvHSSP protein. These results suggested that LvHSSP might promote viral infection in shrimp. Immunocytochemical assay showed that the recombinant LvHSSP protein was located on the membrane of hemocytes. Co-IP results showed that LvHSSP could interact with VP26, the main envelope protein of WSSV, suggesting that LvHSSP might mediate WSSV adhesion and entry into host cells by binding to viral envelope protein. Meanwhile, the total hemocyte counts were significantly decreased after LvHSSP knockdown while increased after supplementing with recombinant LvHSSP protein, supporting the idea of hemocytes as the carrier for systemic dissemination of WSSV. This study reported a novel small protein in hemocytes, which modulated the viral infection in shrimp. Our results will enrich the knowledge of invertebrate innate immunity and provide a new field in the study of hemocyte function.
关键词	a novel small protein hemocyte WSSV infection shrimp
DOI	10.3390/v15010227
收录类别	SCI
语种	英语
资助项目	Key Program of National Natural Science Foundation of China[31830100] ; General Program of National Natural Science Foundation of China[31972829] ; Taishan Scholars Program ; earmarked fund for CARS-48
WOS研究方向	Virology
WOS类目	Virology
WOS记录号	WOS:000918818200001
出版者	MDPI
WOS关键词	PENAEID SHRIMP ; WSSV ; VP26 ; PEPTIDES ; IMMUNITY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183380
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Mingzhe,Li, Shihao,Yu, Yang,et al. A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein[J]. VIRUSES-BASEL,2023,15(1):16.
APA	Sun, Mingzhe,Li, Shihao,Yu, Yang,Zhang, Xiaojun,&Li, Fuhua.(2023).A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein.VIRUSES-BASEL,15(1),16.
MLA	Sun, Mingzhe,et al."A Novel Hemocyte-Specific Small Protein Participates in White Spot Syndrome Virus Infection via Binding to Viral Envelope Protein".VIRUSES-BASEL 15.1(2023):16.