Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea

doi:10.1016/j.seares.2022.102321

	Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea
	Li, Haotian 1,6; Lei, Yanli1,3,5,6 ; Li, Tiegang2,5 ; Saraswat, Rajeev 4
	2023-02-01
发表期刊	JOURNAL OF SEA RESEARCH
ISSN	1385-1101
卷号	191 页码:11
通讯作者	Lei, Yanli(leiyanli@qdio.ac.cn) ; Li, Tiegang(tgli@fio.org.cn)
摘要	The sedimentary archives from the Bering Sea can provide excellent records of the global climate shift. However, paleoclimatic studies from the Bering Sea are limited due to the lack of well calibrated proxies. Various char-acteristics of foraminifera are frequently used as paleoclimatic and paleoceanographic proxies. A precise un-derstanding of the ecology of foraminiferal species is required to use it as proxy. Here, we use next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea. The total genomic deoxyribonucleic acid (DNA) was extracted from 11 surface sediment samples collected from the Bering Sea. The foraminiferal small sub unit (SSU) rDNA was amplified with foraminiferal-specific primers. A total of 2,170,762 effective tags and 1046 operational taxonomic unit (OTUs) were obtained after next -generation sequencing, strict quality control, and data processing. The results showed that the station BR07 had the highest number of OTUs. The highest foraminiferal Margalef index and Shannon-Wiener index were obtained at BR06. Rotaliida accounted for the highest proportion, followed by Textulariida and Monothalamida, with the lowest proportion of Miliolida. From the principal component analysis (PCA), Leptohalysis scottii, Stainforthiidae_X sp., and Rectuvigerina phlegeri were found to be the more important species in the Bering Sea. Based on the redundancy analysis (RDA) and Spearman correlation analysis, we found that the grain size and water depth exhibited significant control on foraminiferal community. The foraminiferal reads were positively correlated with the grain size, whereas the Shannon-Wiener index was negatively correlated with it. The water depth was positively correlated with OTUs, Leptohalysis scottii, and Rectuvigerina phlegeri. The clustering analysis showed that Bering Sea stations cluster together with Yellow Sea stations of similar water depth. The water depth was a bigger factor for foraminiferal community than the geographical distance.
关键词	The Bering Sea Benthic foraminifera Molecular diversity Community composition Next -generation sequencing
DOI	10.1016/j.seares.2022.102321
收录类别	SCI
语种	英语
资助项目	Laoshan Laboratory Science and technology innovation project[LSKJ202204200] ; National Science Foundation of China[41976058] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Wenhai Program of Qingdao Pilot National Laboratory of Marine Science and Technology[2021WHZZB0804]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Marine & Freshwater Biology ; Oceanography
WOS记录号	WOS:000918742000001
出版者	ELSEVIER
WOS关键词	PALEOCEANOGRAPHIC CHANGES ; SURFACE SEDIMENTS ; BAFFIN-BAY ; DIVERSITY ; TEMPERATURE ; PACIFIC ; IMPACT ; ICE ; BIODIVERSITY ; ASSEMBLAGES
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183356
专题	海洋生物分类与系统演化实验室
通讯作者	Lei, Yanli; Li, Tiegang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 2.Minist Nat Resources MNR, Inst Oceanog 1, Key Lab Marine Sedimentol & Environm Geol, Qingdao 266061, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519082, Peoples R China 4.CSIR Natl Inst Oceanog, Geol Oceanog Div, Panaji 403004, Goa, India 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Haotian,Lei, Yanli,Li, Tiegang,et al. Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea[J]. JOURNAL OF SEA RESEARCH,2023,191:11.
APA	Li, Haotian,Lei, Yanli,Li, Tiegang,&Saraswat, Rajeev.(2023).Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea.JOURNAL OF SEA RESEARCH,191,11.
MLA	Li, Haotian,et al."Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea".JOURNAL OF SEA RESEARCH 191(2023):11.