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Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach
Zhou, Li1; Li, Mengna1,2,3; Zhong, Zhaoshan1; Chen, Hao1; Wang, Minxiao1,2; Lian, Chao1; Wang, Hao1; Zhang, Huan1; Cao, Lei1; Li, Chaolun1,2,3,4,5
2023-01-18
Source PublicationFRONTIERS IN MARINE SCIENCE
Volume10Pages:18
Corresponding AuthorLi, Chaolun(lcl@qdio.ac.cn)
AbstractIntroductionMarine metal contamination caused by deep-sea mining activities has elicited great concern from both social and scientific communities. Among the various metals deep-sea organisms might encounter, cadmium (Cd) is a widely detected metal that in very small amounts is nonetheless capable of severe toxicity. Yet due to both remoteness and technical challenges, insights into the effects of metal exposure resulting from mining activities upon deep-sea organisms are limited. MethodsHere, we investigated Cd's toxicological effects on deep-sea mussels of Gigantidas platifrons exposed to 100 or 1000 g/L of Cd for 7 days; an integrated approach was used that incorporated proteomics and metabolomics along with traditional approaches (metal concentrations, metal subcellular distribution, and anti-oxidative and immune-related biochemical indexes). Results and DiscussionResults showed that Cd exposure caused significant Cd's accumulation in mussel gills and redistribution of Cd among subcellular compartments, with cellular debris being the primary binding site. Although anti-oxidative enzymes activities (superoxide dismutase and catalase) were not significantly altered in mussel gills of both exposed groups, the markedly increased level of glutathione S-transferase detected via proteomic technique clearly evinced that deep-sea mussels suffered from oxidative stress under Cd exposure. Besides, altered activities of acid phosphatase and alkaline phosphatase assayed by traditional methods along with the predominant presence of largely altered immune-related proteins detected by proteomic data strongly revealed an immune response of deep-sea mussels elicited by Cd. In addition, results of proteomics combined with those of non-targeted metabolomics demonstrated that Cd could exert toxicity by disrupting cytoskeleton structure, ion homeostasis, and primary metabolisms of energy, lipid, and nucleotide in deep-sea mussels. As demonstrated in this study, proteomics and metabolomics can be used in tandem to provide valuable insights into the molecular mechanisms of deep-sea organisms' response to Cd exposure and for helping to discover potential biomarkers for application during deep-sea mining assessments.
Keywordmetal toxicology Mytilidae deep-sea omics environment monitor
DOI10.3389/fmars.2023.1087411
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology
WOS IDWOS:000920134100001
PublisherFRONTIERS MEDIA SA
WOS KeywordALKALINE-PHOSPHATASE ACTIVITIES ; MYTILUS-GALLOPROVINCIALIS ; BATHYMODIOLUS-AZORICUS ; PROTEIN EXPRESSION ; SUBCELLULAR COMPARTMENTALIZATION ; ENVIRONMENTAL-STRESS ; LIPID-METABOLISM ; HEAVY-METALS ; 2 BIVALVES ; RESPONSES
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/183353
Collection深海极端环境与生命过程研究中心
Corresponding AuthorLi, Chaolun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China
2.Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
First Author AffilicationChinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res
Corresponding Author AffilicationChinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res;  Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Li,Li, Mengna,Zhong, Zhaoshan,et al. Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach[J]. FRONTIERS IN MARINE SCIENCE,2023,10:18.
APA Zhou, Li.,Li, Mengna.,Zhong, Zhaoshan.,Chen, Hao.,Wang, Minxiao.,...&Li, Chaolun.(2023).Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach.FRONTIERS IN MARINE SCIENCE,10,18.
MLA Zhou, Li,et al."Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach".FRONTIERS IN MARINE SCIENCE 10(2023):18.
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