Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach

doi:10.3389/fmars.2023.1087411

	Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach
	Zhou, Li 1; Li, Mengna 1,2,3; Zhong, Zhaoshan1 ; Chen, Hao1 ; Wang, Minxiao 1,2; Lian, Chao 1; Wang, Hao 1; Zhang, Huan 1; Cao, Lei1 ; Li, Chaolun1,2,3,4,5
	2023-01-18
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	10 页码:18
通讯作者	Li, Chaolun(lcl@qdio.ac.cn)
摘要	IntroductionMarine metal contamination caused by deep-sea mining activities has elicited great concern from both social and scientific communities. Among the various metals deep-sea organisms might encounter, cadmium (Cd) is a widely detected metal that in very small amounts is nonetheless capable of severe toxicity. Yet due to both remoteness and technical challenges, insights into the effects of metal exposure resulting from mining activities upon deep-sea organisms are limited. MethodsHere, we investigated Cd's toxicological effects on deep-sea mussels of Gigantidas platifrons exposed to 100 or 1000 g/L of Cd for 7 days; an integrated approach was used that incorporated proteomics and metabolomics along with traditional approaches (metal concentrations, metal subcellular distribution, and anti-oxidative and immune-related biochemical indexes). Results and DiscussionResults showed that Cd exposure caused significant Cd's accumulation in mussel gills and redistribution of Cd among subcellular compartments, with cellular debris being the primary binding site. Although anti-oxidative enzymes activities (superoxide dismutase and catalase) were not significantly altered in mussel gills of both exposed groups, the markedly increased level of glutathione S-transferase detected via proteomic technique clearly evinced that deep-sea mussels suffered from oxidative stress under Cd exposure. Besides, altered activities of acid phosphatase and alkaline phosphatase assayed by traditional methods along with the predominant presence of largely altered immune-related proteins detected by proteomic data strongly revealed an immune response of deep-sea mussels elicited by Cd. In addition, results of proteomics combined with those of non-targeted metabolomics demonstrated that Cd could exert toxicity by disrupting cytoskeleton structure, ion homeostasis, and primary metabolisms of energy, lipid, and nucleotide in deep-sea mussels. As demonstrated in this study, proteomics and metabolomics can be used in tandem to provide valuable insights into the molecular mechanisms of deep-sea organisms' response to Cd exposure and for helping to discover potential biomarkers for application during deep-sea mining assessments.
关键词	metal toxicology Mytilidae deep-sea omics environment monitor
DOI	10.3389/fmars.2023.1087411
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000920134100001
出版者	FRONTIERS MEDIA SA
WOS关键词	ALKALINE-PHOSPHATASE ACTIVITIES ; MYTILUS-GALLOPROVINCIALIS ; BATHYMODIOLUS-AZORICUS ; PROTEIN EXPRESSION ; SUBCELLULAR COMPARTMENTALIZATION ; ENVIRONMENTAL-STRESS ; LIPID-METABOLISM ; HEAVY-METALS ; 2 BIVALVES ; RESPONSES
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183353
专题	深海极端环境与生命过程研究中心
通讯作者	Li, Chaolun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 2.Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
第一作者单位	深海极端环境与生命过程研究中心
通讯作者单位	深海极端环境与生命过程研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Li,Li, Mengna,Zhong, Zhaoshan,et al. Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach[J]. FRONTIERS IN MARINE SCIENCE,2023,10:18.
APA	Zhou, Li.,Li, Mengna.,Zhong, Zhaoshan.,Chen, Hao.,Wang, Minxiao.,...&Li, Chaolun.(2023).Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach.FRONTIERS IN MARINE SCIENCE,10,18.
MLA	Zhou, Li,et al."Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach".FRONTIERS IN MARINE SCIENCE 10(2023):18.