Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes

doi:10.3390/ijms24021672

	Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes
	Deng, Yunyan 1,2,3; Wang, Kui 4; Hu, Zhangxi1,5 ; Hu, Qiang 6; Tang, Yingzhong 1,2,3
	2023
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	24 期号:2 页码:15
通讯作者	Tang, Yingzhong(yingzhong.tang@qdio.ac.cn)
摘要	In numerous studies, researchers have explored the interactions between fungi and their hosting biota in terrestrial systems, while much less attention has been paid to the counterpart interactions in aquatic, and particularly marine, ecosystems. Despite the growing recognition of the potential functions of fungi in structuring phytoplankton communities, the current insights were mostly derived from phytoplankton hosts, such as diatoms, green microalgae, and cyanobacteria. Dinoflagellates are the second most abundant group of phytoplankton in coastal marine ecosystems, and they are notorious for causing harmful algal blooms (HABs). In this study, we used high-throughput amplicon sequencing to capture global snapshots of specific fungal assemblages associated with laboratory-cultured marine dinoflagellate. We investigated a total of 13 clonal cultures of the dinoflagellate Karlodinium veneficum that were previously isolated from 5 geographic origins and have been maintained in our laboratory from several months to more than 14 years. The total recovered fungal microbiome, which consisted of 349 ASVs (amplicon sequencing variants, sequences clustered at a 100% sequence identity), could be assigned to 4 phyla, 18 classes, 37 orders, 65 families, 97 genera, and 131 species. The fungal consortium displayed high diversity and was dominated by filamentous fungi and ascomycetous and basidiomycetous yeasts. A core set of three genera among all the detected fungi was constitutively present in the K. veneficum strains isolated from geographically distant regions, with the top two most abundant genera, Thyridium and Pseudeurotium, capable of using hydrocarbons as the sole or major source of carbon and energy. In addition, fungal taxa previously documented as endophytes in other hosts were also found in all tested strains of K. veneficum. Because host-endophyte interactions are highly variable and strongly case-dependent, these fungal taxa were not necessarily genuine endosymbionts of K. veneficum; instead, it raised the possibility that dinoflagellates could potentially serve as an alternative ecological niche for the colonization of fungal endophytes. Our findings lay the foundation for further investigations into the potential roles or functions of fungi in the regulation of the growth dynamics and HABs of marine dinoflagellates in the field.
关键词	dinoflagellate-linked fungal community endophytic fungi hydrocarbonoclastic microorganisms Karlodinium veneficum marine fungi Thyridium Pseudeurotium
DOI	10.3390/ijms24021672
收录类别	SCI
语种	英语
资助项目	National Science Foundation of China[42176207] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q09] ; Natural Science Foundation of Shenzhen, China[JCYJ20210324094013037]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000917427500001
出版者	MDPI
WOS关键词	AROMATIC-HYDROCARBONS ; UPWELLING ECOSYSTEM ; IDENTIFICATION ; PARASITES ; DYNAMICS ; INFECT ; GEN. ; NOV.
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183329
专题	海洋生态与环境科学重点实验室
通讯作者	Tang, Yingzhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China 5.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China 6.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Fac Synthet Biol,CAS Key Lab Quantitat Engn, Shenzhen 518055, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Deng, Yunyan,Wang, Kui,Hu, Zhangxi,et al. Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(2):15.
APA	Deng, Yunyan,Wang, Kui,Hu, Zhangxi,Hu, Qiang,&Tang, Yingzhong.(2023).Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(2),15.
MLA	Deng, Yunyan,et al."Different Geographic Strains of Dinoflagellate Karlodinium veneficum Host Highly Diverse Fungal Community and Potentially Serve as Possible Niche for Colonization of Fungal Endophytes".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.2(2023):15.