Increasing production and bio-accessibility of natural astaxanthin in Haematococcus pluvialis by screening and culturing red motile cells under high light condition

doi:10.1016/j.biortech.2022.128067

IOCAS-IR > 实验海洋生物学重点实验室

	Increasing production and bio-accessibility of natural astaxanthin in Haematococcus pluvialis by screening and culturing red motile cells under high light condition
	Chen, Feng 1; Xu, Nianjun 1; Liu, Kai1 ; Lv, Rongrong 1; Shi, Jianxin 2; Liu, Jianguo 3; Sun, Xue 1; Hu, Chaoyang 1
	2022-11-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	364 页码:8
通讯作者	Hu, Chaoyang(huchaoyang@nbu.edu.cn)
摘要	The thick cell wall and low astaxanthin productivity were two important bottlenecks limiting industrial production of astaxanthin via Haematococcus pluvialis. This study reports a strategy for increasing production and bio-accessibility of astaxanthin in H. pluvialis by screening and culturing red motile cells under high light condition. Compared with the original strain NBU489, the biomass of the novel isolated strain RMS10 increased by 31.9% under low light condition, and the astaxanthin content (44.6 mg/g) increased by 53.3% after 9-day high light induction, which were readily extracted and digested without cell disruption. Subsequent transcriptomic analysis confirmed the accumulation of astaxanthin and lipids in RMS10 cells as expression of genes associated with biosynthesis of fatty acid and astaxanthin were up-regulated, while those involved in thick cell wall biosynthesis and reactive oxygen species scavenging were down-regulated in RMS10. Collectively, this study provides a simple and effective method for economical production of natural astaxanthin.
关键词	Secondary cell wall Haematococcus pluvialis Transcriptome Wet extraction Simulated gastrointestinal digestion
DOI	10.1016/j.biortech.2022.128067
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0901500] ; National Key Research and Development Program of China[2018YFD0901506] ; Natural Science Foundation of China[31572638] ; Ningbo Major Project of Science and Technology[2021Z114]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000910594400007
出版者	ELSEVIER SCI LTD
WOS关键词	ACCURATE QUANTIFICATION ; ALGA HAEMATOCOCCUS ; DIRECT EXTRACTION ; ACCUMULATION ; GROWTH ; STRESS
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183300
专题	实验海洋生物学重点实验室
通讯作者	Hu, Chaoyang
作者单位	1.Ningbo Univ, Sch Marine Sci, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315832, Peoples R China 2.Shanghai Jiao Tong Univ, Yazhou Bay Inst Deepsea Sci Tech, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab Dev Sci, Shanghai 200240, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, Feng,Xu, Nianjun,Liu, Kai,et al. Increasing production and bio-accessibility of natural astaxanthin in Haematococcus pluvialis by screening and culturing red motile cells under high light condition[J]. BIORESOURCE TECHNOLOGY,2022,364:8.
APA	Chen, Feng.,Xu, Nianjun.,Liu, Kai.,Lv, Rongrong.,Shi, Jianxin.,...&Hu, Chaoyang.(2022).Increasing production and bio-accessibility of natural astaxanthin in Haematococcus pluvialis by screening and culturing red motile cells under high light condition.BIORESOURCE TECHNOLOGY,364,8.
MLA	Chen, Feng,et al."Increasing production and bio-accessibility of natural astaxanthin in Haematococcus pluvialis by screening and culturing red motile cells under high light condition".BIORESOURCE TECHNOLOGY 364(2022):8.