pCO2-driven seawater acidification affects aqueous-phase copper toxicity in juvenile flounder Paralichthys olivaceus: Metal accumulation, antioxidant defenses and detoxification in livers

doi:10.1016/j.scitotenv.2022.160040

	pCO2-driven seawater acidification affects aqueous-phase copper toxicity in juvenile flounder Paralichthys olivaceus: Metal accumulation, antioxidant defenses and detoxification in livers
	Xiao, Zitao 1,3; Cao, Liang 1,2; Liu, Jinhu 1,2; Cui, Wenting 1,3; Dou, Shuozeng 1,2,3,4
	2023-02-01
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	858 页码:13
通讯作者	Dou, Shuozeng(szdou@qdio.ac.cn)
摘要	Ocean acidification potentially influences the biotoxicity of metals and the antioxidant defense systems of marine organisms. This study investigated how pCO2-driven seawater acidification (SA) affected aqueous-phase copper (Cu) toxicity in the juvenile flounder Paralichthys olivaceus from the perspective of hepatic oxidative stress and damage to better understand the mechanisms underlying the biological effects produced by the two stressors. Fish were exposed to aqueous-phase Cu at relevant ambient and polluted concentrations (0, 5, 10, 50, 100 and 200 mu g L-1) at different pH levels (no SA: pH 8.10; moderate SA: pH 7.70, pCO2 similar to 1353.89 mu atm; extreme SA: pH 7.30, pCO2 similar to 3471.27 mu atm) for 28 days. A battery of biomarkers in the livers was examined to investigate their roles in antioxidant defense and detoxification in response to coexposure. Hepatic Cu accumulation (30.22-184.90 mg kg-1) was positively correlated with Cu concentrations. The biomarkers responded adaptively to different redox states following SA and Cu exposure. In unacidified seawater, increases in Cu concentrations significantly induced hepatic lipid peroxidation (LPO, by up to 27.03 %), although compensatory responses in antioxidant defenses and detoxification were activated. Moderate SA helped maintain hepatic redox homeostasis and alleviated LPO through different defense strategies, depending on Cu concentrations. Under extreme SA, antioxidant-based defenses were activated to cope with oxidative stress at ambient-low Cu concentrations but failed to defend against Cu toxicity at polluted Cu levels, and LPO (by up to 63.90 %) was significantly induced. Additionally, thiols (GSH and MT) responded actively to cope with Cu toxicity under SA. SOD, CAT, EROD, and GST were also sensitively involved in defending against hepatic oxidative stress during coexposure. These findings highlight the notable interactive effects of SA and Cu and provide a basis for understanding antioxidant-based defenses in marine fish confronting environmental challenges.
关键词	Ocean acidification Heavy metal Marine fish Oxidative stress Biotransformation Integrative response
DOI	10.1016/j.scitotenv.2022.160040
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[42176107]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000898702900013
出版者	ELSEVIER
WOS关键词	INDUCED OXIDATIVE STRESS ; FRESH-WATER FISH ; OCEAN ACIDIFICATION ; ELEVATED CO2 ; MARINE FISH ; EXPOSURE ; RESPONSES ; BIOMARKERS ; CU ; METALLOTHIONEINS
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183264
专题	海洋生态与环境科学重点实验室
通讯作者	Dou, Shuozeng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xiao, Zitao,Cao, Liang,Liu, Jinhu,et al. pCO2-driven seawater acidification affects aqueous-phase copper toxicity in juvenile flounder Paralichthys olivaceus: Metal accumulation, antioxidant defenses and detoxification in livers[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,858:13.
APA	Xiao, Zitao,Cao, Liang,Liu, Jinhu,Cui, Wenting,&Dou, Shuozeng.(2023).pCO2-driven seawater acidification affects aqueous-phase copper toxicity in juvenile flounder Paralichthys olivaceus: Metal accumulation, antioxidant defenses and detoxification in livers.SCIENCE OF THE TOTAL ENVIRONMENT,858,13.
MLA	Xiao, Zitao,et al."pCO2-driven seawater acidification affects aqueous-phase copper toxicity in juvenile flounder Paralichthys olivaceus: Metal accumulation, antioxidant defenses and detoxification in livers".SCIENCE OF THE TOTAL ENVIRONMENT 858(2023):13.