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Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins
Yu, Chuandi1,2; Ding, Wen3; Zhu, Lei; Zhou, Yuhang1,4; Dong, Yingkui1,5; Li, Ling1; Liu, Juanjuan; Wang, Yizhuo2; Li, Zehua2; Zhu, Lina2; Chen, FaJun; Ruan, Maosen; Qian, Dongming6; Wang, Yujuan; Wu, Bo; Xu, Huangtao; Li, Ming1,2; Bi, Yunchen7,8; Wang, Hao7,8; Wang, Weiqian9; Chao, Peng9; Xing, Lei1; Shen, Bing3; Dai, Han; Zha, Lisha4; Zhao, Hongxin1,6; Wang, Junfeng1,2,5
2023-01-20
Source PublicationISCIENCE
Volume26Issue:1Pages:21
Corresponding AuthorZha, Lisha(zhalisha@ahau.edu.cn) ; Zhao, Hongxin(zhx@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn)
AbstractInfluenza A virus poses a constant challenge to human health. The highly conserved influenza matrix-2 (M2) protein is an attractive target for the development of a universal antibody-based drug. However, screening using antigens with subphysiological conformation in a nonmembrane environment significantly reduces the generation of efficient antibodies. Here, M2(1-46) was incorporated into nanodiscs (M2-nanodiscs) with M2 in a membrane-embedded tetrameric conformation, closely resembling its natural physiological state in the influenza viral envelope. M2-nanodisc generation, an antigen, was followed by Chiloscyllium plagiosum immunization. The functional vNARs were selected by phage display panning strategy from the shark immune library. One of the isolated vNARs, AM2H10, could specifically bind to tetrameric M2 instead of monomeric M2e (the ectodomain of M2 protein). Furthermore, AM2H10 blocked ion influx through amantadine-sensitive and resistant M2 channels. Our findings indicated the possibility of developing functional shark nanobodies against various influenza viruses by targeting the M2 protein.
DOI10.1016/j.isci.2022.105736
Indexed BySCI
Language英语
Funding ProjectAnhui Technologies Major Program[201903a07020026] ; Chinese Academy of Sciences[YZJJZX202004] ; Users with Excellence Program of Hefei Science Center,CAS[2020HSC-UE018] ; National Natural Science Foundation of China[41876168] ; Taishan Young Scholar Program of Shandong Province[tsqn201812108]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000901534700006
PublisherCELL PRESS
WOS KeywordINTEGRAL MEMBRANE-PROTEINS ; SINGLE-DOMAIN ; MONOCLONAL-ANTIBODY ; LIPID-BILAYERS ; A VIRUSES ; V-REGION ; SHARK ; CHANNEL ; GENERATION ; SELECTION
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/183261
Collection实验海洋生物学重点实验室
Corresponding AuthorZha, Lisha; Zhao, Hongxin; Wang, Junfeng
Affiliation1.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
3.Anhui Med Univ, Sch Basic Med Sci, Hefei 230032, Anhui, Peoples R China
4.Anhui Agr Univ, Int Immunol Ctr, Hefei 230036, Anhui, Peoples R China
5.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230039, Anhui, Peoples R China
6.Hefei China Sci Longwood Biol Technol Co Ltd, Hefei 230088, Anhui, Peoples R China
7.Chinese Acad Sci, Inst Oceanol, Ctr OceanMega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
8.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Biol & Biotechnol Lab, Qingdao 266237, Shandong, Peoples R China
9.Chinese Acad Sci, Shanghai Adv Res Inst, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China
Recommended Citation
GB/T 7714
Yu, Chuandi,Ding, Wen,Zhu, Lei,et al. Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins[J]. ISCIENCE,2023,26(1):21.
APA Yu, Chuandi.,Ding, Wen.,Zhu, Lei.,Zhou, Yuhang.,Dong, Yingkui.,...&Wang, Junfeng.(2023).Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins.ISCIENCE,26(1),21.
MLA Yu, Chuandi,et al."Screening and characterization of inhibitory vNAR targeting nanodisc-assembled influenza M2 proteins".ISCIENCE 26.1(2023):21.
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