The Toll pathway and Duox-ROS system are required for the clam antibacterial immune response in the hepatopancreas

doi:10.1016/j.aquaculture.2023.739637

IOCAS-IR > 实验海洋生物学重点实验室

	The Toll pathway and Duox-ROS system are required for the clam antibacterial immune response in the hepatopancreas
	Yu, Jiajia1,2 ; Teng, Shuangshuang 3; Yue, Xin 1; Wang, Hongxia 1; Liu, Baozhong1
	2023-09-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	574 页码:10
通讯作者	Wang, Hongxia(hxwang@qdio.ac.cn)
摘要	Vibriosis is one of the most important bacterial diseases that can cause mass mortality of mollusks, especially in summer, and has contributed to dramatic economic losses. Infection is the result of complex molecular interactions between the host and invading agents. Understanding the interaction mechanism could provide a scientific basis for disease control and genetic breeding of resistant varieties. In this paper, the clam Meretrix petechialis was challenged with Vibrio parahaemolyticus to simulate bacterial infection in the natural environment. The Vibrio load variation in the clam hepatopancreas was surveyed during the early phase of Vibrio infection (i.e., latency, prodrome), and the results showed that the Vibrio load was high at 1 dpi (days post-infection), and then dramatically decreased at 2 dpi and remained low at the following time points. The key genes in Toll pathway and the reactive oxygen species (ROS) system were activated to release antibacterial peptide (AMP) and H2O2 during early bacterial infection, respectively. An RNAi experiment further supported that the Toll pathway and ROS system could directly control Vibrio abundance by producing AMP and H2O2. This study provides novel insights into the identity of host factors that enhance or restrict pathogenesis.
关键词	Meretrix petechialis Vibrio parahaemolyticus Vibrio load Toll pathway ROS system
DOI	10.1016/j.aquaculture.2023.739637
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Pro-gram of the Chinese Academy of Sciences[XDA24030304] ; Key Research and Development Program of Shandong[ZR2020MD089] ; Natural Science Foundation of Shandong Province[CARS-49] ; Taishan Industrial Leading Talents Project to Shandong Dehe Mingxing Biotechnology Co., Ltd. ; [2021LZGC029]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001001344100001
出版者	ELSEVIER
WOS关键词	IN-SITU HYBRIDIZATION ; MOLECULAR CHARACTERIZATION ; INNATE IMMUNITY ; MANILA CLAM ; RUDITAPES-PHILIPPINARUM ; MERETRIX-PETECHIALIS ; BACTERIAL-INFECTION ; RIBOSOMAL-RNA ; DROSOPHILA ; HOMEOSTASIS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183248
专题	实验海洋生物学重点实验室
通讯作者	Wang, Hongxia
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 3.Zhejiang Mariculture Res Inst, Zhejiang Key Lab Exploitat, Preservat Coastal Bioresource Wenzhou Key Lab Mari, Wenzhou 325005, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yu, Jiajia,Teng, Shuangshuang,Yue, Xin,et al. The Toll pathway and Duox-ROS system are required for the clam antibacterial immune response in the hepatopancreas[J]. AQUACULTURE,2023,574:10.
APA	Yu, Jiajia,Teng, Shuangshuang,Yue, Xin,Wang, Hongxia,&Liu, Baozhong.(2023).The Toll pathway and Duox-ROS system are required for the clam antibacterial immune response in the hepatopancreas.AQUACULTURE,574,10.
MLA	Yu, Jiajia,et al."The Toll pathway and Duox-ROS system are required for the clam antibacterial immune response in the hepatopancreas".AQUACULTURE 574(2023):10.