Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice

doi:10.1016/j.heliyon.2023.e15197

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice
	Li, Zhi1,2,3 ; Zhang, Quanbin 1,2; Sun, Yuan-yuan1 ; Wu, Ning 1,2,4,5
	2023-04-01
发表期刊	HELIYON
卷号	9 期号:4 页码:9
通讯作者	Sun, Yuan-yuan(sunyuanyuan@qdio.ac.cn) ; Wu, Ning(wuning@qdio.ac.cn)
摘要	Glycocalyx is located outside the vascular endothelial cells playing an important role in vascular homeostasis. However, lacking efficient detection methods is one of the biggest obstacles to study the glycocalyx. In this study, three dehydration methods were used to compare the preservation of HUVEC, aorta and kidney glycocalyx by transmission electron microscope. The chemical pre -fixation was performed by lanthanum nitrate staining, and the mice aorta and renal glycocalyx were prepared by different dehydration methods such as ethanol gradient, acetone gradient and low temperature dehydration. HUVEC glycocalyx was prepared by acetone gradient and low temperature dehydration. Low temperature dehydration method preserves HUVEC and mice aortic glycocalyx completely, which had a certain thickness and presented a needle-like structure. But for mice kidney, the acetone gradient dehydration preparation method could better preserve the glycocalyx integrity than other two methods. In conclusion, low temperature dehydration method is suitable for HUVEC and aortic glycocalyx preservation, acetone gradient dehydration method is more suitable for kidney glycocalyx preservation.
关键词	Glycocalyx Dehydration Transmission electron microscopy
DOI	10.1016/j.heliyon.2023.e15197
收录类别	SCI
语种	英语
资助项目	Innovative Research Group Project of the National Natural Science Foundation of China[42176137] ; Nantong Science and Technology Project, China[MS12021037]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000999832300001
出版者	CELL PRESS
WOS关键词	ENDOTHELIAL GLYCOCALYX ; DYSFUNCTION ; CELL ; SULFATE ; BIOLOGY ; LAYER
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183166
专题	实验海洋生物学重点实验室
通讯作者	Sun, Yuan-yuan; Wu, Ning
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Dept Drugs & Bioprod, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Nantong Zhongke Marine Sci & Technol Res & Dev Ctr, Nantong, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Zhi,Zhang, Quanbin,Sun, Yuan-yuan,et al. Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice[J]. HELIYON,2023,9(4):9.
APA	Li, Zhi,Zhang, Quanbin,Sun, Yuan-yuan,&Wu, Ning.(2023).Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice.HELIYON,9(4),9.
MLA	Li, Zhi,et al."Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice".HELIYON 9.4(2023):9.