Metabarcoding analysis identifies high diversity of harmful algal bloom species in the coastal waters of the Beibu Gulf

doi:10.1002/ece3.10127

	Metabarcoding analysis identifies high diversity of harmful algal bloom species in the coastal waters of the Beibu Gulf
	He, Liyan 1,2,3; Yu, Zhiming1,2,3,4 ; Xu, Xin1,2,3 ; Zhu, Jianan 1,2,3; Yuan, Yongquan1,2,3 ; Cao, Xihua1,2,3 ; Song, Xiuxian1,2,3,4
	2023-05-01
发表期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
卷号	13 期号:5 页码:16
通讯作者	Yu, Zhiming(zyu@qdio.ac.cn)
摘要	Harmful algal blooms (HABs) have occurred more frequently in recent years. In this study, to investigate their potential impact in the Beibu Gulf, short-read and long-read metabarcoding analyses were combined for annual marine phytoplankton community and HAB species identification. Short-read metabarcoding showed a high level of phytoplankton biodiversity in this area, with Dinophyceae dominating, especially Gymnodiniales. Multiple small phytoplankton, including Prymnesiophyceae and Prasinophyceae, were also identified, which complements the previous lack of identifying small phytoplankton and those unstable after fixation. Of the top 20 phytoplankton genera identified, 15 were HAB-forming genera, which accounted for 47.3%-71.5% of the relative abundance of phytoplankton. Based on long-read metabarcoding, a total of 147 OTUs (PID > 97%) belonging to phytoplankton were identified at the species level, including 118 species. Among them, 37 species belonged to HAB-forming species, and 98 species were reported for the first time in the Beibu Gulf. Contrasting the two metabarcoding approaches at the class level, they both showed a predominance of Dinophyceae, and both included high abundances of Bacillariophyceae, Prasinophyceae, and Prymnesiophyceae, but the relative contents of the classes varied. Notably, the results of the two metabarcoding approaches were quite different below the genus level. The high abundance and diversity of HAB species were probably due to their special life history and multiple nutritional modes. Annual HAB species variation revealed in this study provided a basis for evaluating their potential impact on aquaculture and even nuclear power plant safety in the Beibu Gulf.
关键词	Beibu Gulf diversity harmful algal species long-read metabarcoding short-read
DOI	10.1002/ece3.10127
收录类别	SCI
语种	英语
资助项目	Laoshan Laboratory[LSKJ202203700] ; National Key Research and Development Program of China[2022YFC3106004] ; Taishan Program of Shandong Province ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-03]
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Ecology ; Evolutionary Biology
WOS记录号	WOS:000993578900001
出版者	WILEY
WOS关键词	PHYTOPLANKTON COMMUNITY ; DINOFLAGELLATE ; MIXOTROPHY ; PICOEUKARYOTES ; DINOPHYCEAE ; NUMBER ; DNA
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183130
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, Zhiming
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Laoshan Lab, Funct Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	He, Liyan,Yu, Zhiming,Xu, Xin,et al. Metabarcoding analysis identifies high diversity of harmful algal bloom species in the coastal waters of the Beibu Gulf[J]. ECOLOGY AND EVOLUTION,2023,13(5):16.
APA	He, Liyan.,Yu, Zhiming.,Xu, Xin.,Zhu, Jianan.,Yuan, Yongquan.,...&Song, Xiuxian.(2023).Metabarcoding analysis identifies high diversity of harmful algal bloom species in the coastal waters of the Beibu Gulf.ECOLOGY AND EVOLUTION,13(5),16.
MLA	He, Liyan,et al."Metabarcoding analysis identifies high diversity of harmful algal bloom species in the coastal waters of the Beibu Gulf".ECOLOGY AND EVOLUTION 13.5(2023):16.