A novel teleost microRNA regulates autophagy and NF-KB activation during bacterial infection

doi:10.1016/j.fsi.2023.108778

IOCAS-IR > 实验海洋生物学重点实验室

	A novel teleost microRNA regulates autophagy and NF-KB activation during bacterial infection
	Zhang, Zhanwei 1,2; Guan, Xiaolu 2; Sun, Li 2,3,4
	2023-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	137 页码:8
通讯作者	Guan, Xiaolu(guanxiaolu@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
摘要	MicroRNAs (miRNAs) are a class of non-coding RNAs with regulatory functions in many cellular processes, including immune defense. In this study, we identified novel-m0089-3p, a novel miRNA with unknown function, in the teleost fish Japanese flounder (Paralichthys olivaceus) and investigated its immune function. Novel-m0089 -3p was found to target the autophagy-associated gene ATG7 and negatively regulate ATG7 expression via interaction with the 3' UTR of ATG7. During the infection of the bacterial pathogen Edwardsiella tarda, novel -m0089-3p expression was induced in flounder, which in turn repressed ATG7 expression. Overexpression of novel-m0089-3p or blocking ATG7 expression inhibited autophagy and promoted the intracellular replication of E. tarda. Novel-m0089-3p overexpression, as well as E. tarda infection, activated NF -KB and stimulated the expression of inflammatory cytokines. Together these results revealed an important role of novel-m0089-3p in response to bacterial infection.
关键词	MicroRNA Paralichthys olivaceus Bacterial infection Autophagy Immune regulation
DOI	10.1016/j.fsi.2023.108778
收录类别	SCI
语种	英语
资助项目	Shandong Provincial Natural Science Foundation[ZR2021QC124] ; Laboratory for Marine Biology and Biotechnology[OF2019NO08] ; Pilot National Laboratory for Marine Science and Technology (Qingdao) ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000993886600001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS关键词	EDWARDSIELLA-TARDA ; COMPREHENSIVE IDENTIFICATION ; ARCHITECTURE ; INFLAMMATION ; MECHANISM ; PROTEINS ; GROUPER ; MIRNAS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183123
专题	实验海洋生物学重点实验室
通讯作者	Guan, Xiaolu; Sun, Li
作者单位	1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Zhanwei,Guan, Xiaolu,Sun, Li. A novel teleost microRNA regulates autophagy and NF-KB activation during bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2023,137:8.
APA	Zhang, Zhanwei,Guan, Xiaolu,&Sun, Li.(2023).A novel teleost microRNA regulates autophagy and NF-KB activation during bacterial infection.FISH & SHELLFISH IMMUNOLOGY,137,8.
MLA	Zhang, Zhanwei,et al."A novel teleost microRNA regulates autophagy and NF-KB activation during bacterial infection".FISH & SHELLFISH IMMUNOLOGY 137(2023):8.