Differential spatial-temporal dynamics of Alexandrium species revealed using metabarcoding analysis by the 18S rDNA V4 region in Jiaozhou Bay, China

doi:10.1007/s10811-023-02970-9

	Differential spatial-temporal dynamics of Alexandrium species revealed using metabarcoding analysis by the 18S rDNA V4 region in Jiaozhou Bay, China
	Ding, Xiangxiang1,2,3,4 ; Liu, Shuya 1,2,4; Cui, Zongmei 1,2,4; Zhao, Yongfang 2,4,5; Chen, Nansheng 1,2,4,6
	2023-04-28
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
页码	16
通讯作者	Chen, Nansheng(chenn@qdio.ac.cn)
摘要	The dinoflagellate genus Alexandrium hosts many toxigenic species with remarkable negative influence on ecosystems and human health by developing harmful algal blooms (HABs). Despite 34 Alexandrium species having been characterized with unique taxonomical features, relatively few Alexandrium species have been identified and traced in ecological studies, largely due to the limited resolution of morphology-based methodologies and demanding workload associated with ecological studies. Thus, both diversity and spatial-temporal dynamics of Alexandrium species in marine ecosystems remain poorly resolved and understood, leading to severe under appreciation of Alexandrium species in ecosystems. In this project, metabarcoding of the 18S rDNA V4 region was applied for the first time to quantitatively ascertain biodiversity and spatial-temporal dynamics of Alexandrium species in Jiaozhou Bay, a semi-enclosed model ecosystem in Qingdao, China. Eight known Alexandrium species (A. affine, A. andersonii, A. catenella, A. leei, A. minutum, A. ostenfeldii, A. pacificum, and A. pohangense), one unidentified Alexandrium species, and possibly three additional Alexandrium species (A. hiranoi, A. pseudogonyaulax, A. insuetum) were successfully identified in this study, compared to only four previously reported Alexandrium species in Jiaozhou Bay, demonstrating the competitive strength of metabarcoding technology in ecological research of Alexandrium species. Quantitative analysis showed that most Alexandrium species displayed strong temporal dynamics with high abundance in months with relative high temperature. In particular, Alexandrium affine displayed a strong preference from June to December, peaking in September, suggesting that temperature is an important driving factor for the temporal dynamics of Alexandrium species. However, Alexandrium catenella displayed preference from January to May. Metabarcoding analysis could be widely applied for detection and study of spatial-temporal dynamics of Alexandrium species.
关键词	Alexandrium Dinoflagellate Metabarcoding analysis Jiaozhou Bay Harmful algal bloom Biodiversity Spatial-temporal dynamics
DOI	10.1007/s10811-023-02970-9
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000980146300003
出版者	SPRINGER
WOS关键词	COASTAL WATERS ; PHYTOPLANKTON COMMUNITY ; OSTENFELDII DINOPHYCEAE ; EUKARYOTIC COMMUNITY ; MOLECULAR DIVERSITY ; HIROSHIMA BAY ; BOHAI SEA ; TAMARENSE ; FUNDYENSE ; TOXICITY
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183093
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 10039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China 6.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Ding, Xiangxiang,Liu, Shuya,Cui, Zongmei,et al. Differential spatial-temporal dynamics of Alexandrium species revealed using metabarcoding analysis by the 18S rDNA V4 region in Jiaozhou Bay, China[J]. JOURNAL OF APPLIED PHYCOLOGY,2023:16.
APA	Ding, Xiangxiang,Liu, Shuya,Cui, Zongmei,Zhao, Yongfang,&Chen, Nansheng.(2023).Differential spatial-temporal dynamics of Alexandrium species revealed using metabarcoding analysis by the 18S rDNA V4 region in Jiaozhou Bay, China.JOURNAL OF APPLIED PHYCOLOGY,16.
MLA	Ding, Xiangxiang,et al."Differential spatial-temporal dynamics of Alexandrium species revealed using metabarcoding analysis by the 18S rDNA V4 region in Jiaozhou Bay, China".JOURNAL OF APPLIED PHYCOLOGY (2023):16.