Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii

doi:10.1016/j.ijbiomac.2023.123176

IOCAS-IR > 实验海洋生物学重点实验室

	Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii
	Li, Rongfeng 1,2; Yu, Huahua1,2 ; Li, Aoyu 1,3; Yu, Chunlin1,2 ; Li, Pengcheng1,2
	2023-03-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	230 页码:8
通讯作者	Li, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
摘要	Jellyfish Cyanea nozakii venom is a complex mixture of various toxins, most of which are proteinous biological macromolecules and are considered to be responsible for clinical symptoms or even death after a severe sting. Previous transcriptome and proteome analysis identified hundreds of toxins in the venom, including hemolysins, C-type lectin, phospholipase A2, potassium channel inhibitor, metalloprotease, etc. However, it is not clear which toxin in the venom plays the most important role in lethality. Herein, we isolated the key lethal toxin (Letoxcn) from jellyfish Cyanea nozakii using anion exchange chromatography, size-exclusion chromatography, and cation exchange chromatography. The molecular weight of Letoxcn is similar to 50 kDa with the N-terminal sequences of QADAEKVNLPVGVCV. Peptide mass fingerprinting analysis of Letoxcn shows that it may have some motifs of phospholipase, metalloproteinase, thrombin-like enzyme, potassium channel toxin, etc. However, only metalloproteinase activity but no hemolytic, PLA2, or blood coagulation activity was observed from in vitro toxicity analysis. Overall, this study uncovered and characterized the key lethal toxin in the venom of jellyfish Cyanea nozakii, which will not only help to reveal the molecule mechanism of the lethality, but also develop effective treatment like antivenom for this jellyfish sting in the future.
关键词	Jellyfish Venom Toxin Letoxcn Cyanea nozakii
DOI	10.1016/j.ijbiomac.2023.123176
收录类别	SCI
语种	英语
资助项目	National Natural Science Founda-tion of China[41876164] ; Natural Science Foundation of Shandong Province[ZR2019QD012]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000990027600001
出版者	ELSEVIER
WOS关键词	BOX JELLYFISH ; PARTIAL-PURIFICATION ; CHIRONEX-FLECKERI ; SEA WASP ; VENOM PROTEINS ; STINGS ; NEUROTOXIN ; MANAGEMENT ; HEMOLYSIN ; TOXICITY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183003
专题	实验海洋生物学重点实验室
通讯作者	Li, Rongfeng; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Rongfeng,Yu, Huahua,Li, Aoyu,et al. Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,230:8.
APA	Li, Rongfeng,Yu, Huahua,Li, Aoyu,Yu, Chunlin,&Li, Pengcheng.(2023).Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,230,8.
MLA	Li, Rongfeng,et al."Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 230(2023):8.