ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp

doi:10.1016/j.scitotenv.2023.163239

IOCAS-IR > 实验海洋生物学重点实验室

	ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp
	Zhang, Xiaoxi1,2 ; Zhang, Xiaojun 1,2,3,4; Yuan, Jianbo 1,2,3,4; Li, Fuhua 1,2,3,4
	2023-07-01
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	880 页码:12
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
摘要	The a-crystallin domain-containing (ACD-containing) gene family, which includes typical small heat shock proteins (sHSPs), is the most ubiquitous and diverse family of putative chaperones in all organisms, including eukaryotes and prokaryotes. In the present study, approximately 54-117 ACD-containing genes were identified in five penaeid shrimp species, yielding a significant expansion in comparison with other crustaceans (generally 6-20 ACDcontaining genes). Unlike typical sHSPs, which contain a single ACD domain, the ACD-containing genes of penaeid shrimp contain additional ACD domains (3-7 domains, in general), thus having a larger molecular weight and a more complex 3D structure. As indicated by the RNA-seq and qRT-PCR results, the ACD-containing genes of penaeid shrimp showed a strong response to high temperatures. Furthermore, heterologous expression and citrate synthase assays of three representative ACD-containing genes confirmed that their chaperone activity could enhance the thermotolerance of E. coli and prevent the aggregation of substrate proteins at high temperatures. Compared with penaeid shrimp species with a relatively low thermo-tolerance (Fenneropenaeus chinensis and Marsupenaeus japonicus), the species with high thermo-tolerance (Litopenaeus vannamei and Fenneropenaeus indicus) contained more ACD-containing genes due to tandem duplication and exhibited biased expression levels under high temperatures. This can explain the divergent thermo-tolerance of different penaeid shrimp species. In conclusion, the ACD-containing genes in penaeid shrimp could be assigned as new chaperones and contribute to their divergent thermo-tolerance phenotypes and adaptations to the ecological environment.
关键词	ACD-containing genes Chaperones Divergent thermo-tolerance Penaeid shrimp Gene family expansion Adaptation
DOI	10.1016/j.scitotenv.2023.163239
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0900103] ; National Natural Science Foundation of China[42206091] ; China Postdoctoral Science Foundation[2021M703249] ; Agriculture Research System-48 of China[CARS-48] ; Taishan Scholars Program ; Oceanographic Data Center, IOCAS
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000985387000001
出版者	ELSEVIER
WOS关键词	ALPHA-CRYSTALLIN DOMAIN ; HEAT-SHOCK PROTEINS ; LITOPENAEUS-VANNAMEI ; TEMPERATURE ; PACIFIC ; ALIGNMENT ; STRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182940
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,et al. ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,880:12.
APA	Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,&Li, Fuhua.(2023).ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp.SCIENCE OF THE TOTAL ENVIRONMENT,880,12.
MLA	Zhang, Xiaoxi,et al."ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp".SCIENCE OF THE TOTAL ENVIRONMENT 880(2023):12.