The Isolation of Anaerobic and Facultative Anaerobic Sulfate-Reducing Bacteria (SRB) and a Comparison of Related Enzymes in Their Sulfate Reduction Pathways

doi:10.3390/microorganisms11082019

	The Isolation of Anaerobic and Facultative Anaerobic Sulfate-Reducing Bacteria (SRB) and a Comparison of Related Enzymes in Their Sulfate Reduction Pathways
	Wang, Jing 1,2; Li, Xiaohong 1; Guan, Fang2,3 ; Yang, Zhibo 1; Zhai, Xiaofan2,3 ; Zhang, Yimeng 2,3; Tang, Xuexi 1,3; Duan, Jizhou 2,3; Xiao, Hui 1,3
	2023-08-01
发表期刊	MICROORGANISMS
卷号	11 期号:8 页码:14
通讯作者	Duan, Jizhou(duanjz@qdio.ac.cn) ; Xiao, Hui(xiaohui@ouc.edu.cn)
摘要	Sulfate-reducing bacteria (SRB) are an important group of microorganisms that cause microbial corrosion. In this study, culturable SRB were isolated and identified from the inner rust layer of three kinds of steel and from sediments, and a comparison of amino acid sequences encoding related enzymes in the sulfate reduction pathway between anaerobic and facultative anaerobic SRB strains was carried out. The main results are as follows. (1) Seventy-seven strains were isolated, belonging to five genera and seven species, with the majority being Desulfovibrio marinisediminis. For the first time, Holodesulfovibrio spirochaetisodalis and Acinetobacter bereziniae were separated from the inner rust layer of metal, and sulfate reduction by A. bereziniae, Virgibacillus dokdonensis, and Virgibacillus chiguensis, etc., was also demonstrated for the first time. (2) Three strains of strictly anaerobic bacteria and four strains of facultative anaerobic bacteria were screened from seven bacterial strains. (3) Most of the anaerobic SRB only contained enzymes for the dissimilatory sulfate reduction pathway, while those of facultative anaerobic bacteria capable of producing hydrogen sulfide included two possible ways: containing the related enzymes from the dissimilatory pathway only, or containing enzymes from both dissimilatory and assimilation pathways. This study newly discovered that some bacterial genera exhibit sulfate reduction ability and found that there are differences in the distribution of enzymes related to the sulfate reduction pathway between anaerobic and facultative anaerobic SRB type trains, providing a basis for the development and utilization of sulfate-reducing bacterial resources and furthering our understanding of the metabolic mechanisms of SRB.
关键词	inner rust layer and sediment sulfate-reducing bacteria anaerobic SRB facultative anaerobic bacteria sulfate reduction pathway
DOI	10.3390/microorganisms11082019
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42076044]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001056333600001
出版者	MDPI
WOS关键词	MICROBIOLOGICALLY INFLUENCED CORROSION ; SP NOV. ; MICROBIAL COMMUNITIES ; MICROORGANISMS ; PHYSIOLOGY ; DYNAMICS ; BIOFILMS ; OIL
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182886
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Duan, Jizhou; Xiao, Hui
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Laoshan Lab, Qingdao 266000, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Jing,Li, Xiaohong,Guan, Fang,et al. The Isolation of Anaerobic and Facultative Anaerobic Sulfate-Reducing Bacteria (SRB) and a Comparison of Related Enzymes in Their Sulfate Reduction Pathways[J]. MICROORGANISMS,2023,11(8):14.
APA	Wang, Jing.,Li, Xiaohong.,Guan, Fang.,Yang, Zhibo.,Zhai, Xiaofan.,...&Xiao, Hui.(2023).The Isolation of Anaerobic and Facultative Anaerobic Sulfate-Reducing Bacteria (SRB) and a Comparison of Related Enzymes in Their Sulfate Reduction Pathways.MICROORGANISMS,11(8),14.
MLA	Wang, Jing,et al."The Isolation of Anaerobic and Facultative Anaerobic Sulfate-Reducing Bacteria (SRB) and a Comparison of Related Enzymes in Their Sulfate Reduction Pathways".MICROORGANISMS 11.8(2023):14.