A neural cell adhesion molecule from oyster Crassostrea gigas: Molecular identification and immune functional characterization

doi:10.1016/j.ijbiomac.2023.125756

IOCAS-IR > 实验海洋生物学重点实验室

	A neural cell adhesion molecule from oyster Crassostrea gigas: Molecular identification and immune functional characterization
	Qiao, Xue 1,4; Liu, Conghui2 ; Wang, Weilin1,2,3,4 ; Yang, Chuanyan 1,2,4; Li, Meijia1,2 ; Yi, Qilin 1,2,3,4; Kong, Ning1,2,3,4 ; Qiu, Limei 2; Liu, Xiyang 1,4; Wang, Lingling 1,2,3,4,5; Song, Linsheng 1,2,3,4,5
	2023-08-30
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	247 页码:13
通讯作者	Wang, Lingling(wanglingling@dlou.edu.cn) ; Song, Linsheng(lshsong@dlou.edu.cn)
摘要	Neural cell adhesion molecules (NCAMs) are large cell-surface glycoproteins playing important roles in cell-cell and cell-extracellular matrix interactions in nervous system. Recent study identified a homologue of NCAM (CgNCAM) from the Pacific oyster Crassostrea gigas. Its ORF was of 2634 bp which encodes a protein (877 amino acids) consisting of five immunoglobulin domains and two fibronectin type III domains. CgNCAM transcripts were broadly distributed in oyster tissues especially in mantle, labial palp and haemolymph. CgNCAM showed up-regulated expression in haemocytes of oysters after Vibrio splendidus and Staphylococcus aureus stimulation. The recombinant CgNCAM protein (rCgNCAM) was able to bind manose, lipopolysaccharide and glucan, as well as different microbes including Gram-negative bacteria and fungi. rCgNCAM displayed bacterial agglutination and hemagglutination activity. CgNCAM improved the phagocytosis of haemocytes towards V. splendidus by regulating the expression of CgIntegrin, CgRho J and CgMAPKK. Moreover, CgNCAM was involved in the extracellular trap establishment of haemocytes after V. splendidus stimulation. The results collectively indicated that CgNCAM acted as a recognition receptor executing multiple immune functions to recognize and eliminate invading microorganisms in innate immunity of oysters.
关键词	IgCAM Neural cell adhesion molecule PRR Phagocytosis
DOI	10.1016/j.ijbiomac.2023.125756
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41961124009] ; National Natural Science Foundation of China[32230110] ; National Key Research and Development Program[2018YFD0900502] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research from MARA ; innovation team of Aquaculture Environment Safety from Liaoning Province[XLYC1902012] ; earmarked fund[CARS-49] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research from MARA ; Distinguished Professor of Liaoning[XLYC1902012] ; Climbing Scholar of Liaoning ; innovation team of Aquaculture Environment Safety from Liaoning Province[LT202009] ; Dalian High Level Talent Innovation Support Program[2022RG14]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001048780500001
出版者	ELSEVIER
WOS关键词	PATTERN-RECOGNITION RECEPTOR ; TYROSINE KINASE TRKB ; IMMUNOGLOBULIN SUPERFAMILY ; VIBRIO-SPLENDIDUS ; BINDING-SITE ; NITRIC-OXIDE ; NCAM ; EXPRESSION ; PROTEIN ; PHAGOCYTOSIS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182826
专题	实验海洋生物学重点实验室
通讯作者	Wang, Lingling; Song, Linsheng
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266235, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Control, Dalian 116023, Peoples R China 5.Dalian Ocean Univ, Dalian 116023, Peoples R China
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Qiao, Xue,Liu, Conghui,Wang, Weilin,et al. A neural cell adhesion molecule from oyster Crassostrea gigas: Molecular identification and immune functional characterization[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,247:13.
APA	Qiao, Xue.,Liu, Conghui.,Wang, Weilin.,Yang, Chuanyan.,Li, Meijia.,...&Song, Linsheng.(2023).A neural cell adhesion molecule from oyster Crassostrea gigas: Molecular identification and immune functional characterization.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,247,13.
MLA	Qiao, Xue,et al."A neural cell adhesion molecule from oyster Crassostrea gigas: Molecular identification and immune functional characterization".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 247(2023):13.