Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value

doi:10.1016/j.algal.2023.103204

IOCAS-IR > 实验海洋生物学重点实验室

	Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value
	Guo, Menglin 1,2,3; Shao, Zhizhuo 1,2,3; Zheng, Zhenbing 1,2; Wang, Guangce 1,2
	2023-07-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	74 页码:9
通讯作者	Zheng, Zhenbing(zbzheng@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Ferredoxin-NADP+ oxidoreductase (FNR) is involved in reducing Fd in linear electron transportation (LET) in chloroplast. While the oxidized pentose phosphate pathway (OPPP) occurred in cytoplasm has close relationship with cyclic electron transportation (CET) under stress condition. However, whether FNR and OPPP cooperate with each other, and in what way they are associated remains unclear. In this study, Neopyropia yezoensis, a strong resurrection red macroalga, was used to study the relationship between FNR and OPPP. FNR genetic transformation strains of N. yezoensis, fnrOE (overexpression) and fnrSi (silence) were obtained. The results showed that the growth rate of fnrSi was reduced remarkably. The expression level of genes encoding key en-zymes in OPPP and enzyme activity of G6PDH were up regulated in transgenic strains, indicating the enhancement of OPPP. Besides, the NADPH and NADP+ content in WT and transgenic lines were also measured. Y (I) of both fnrOE and fnrSi decreased significantly under dark treatment, indicating that FNR content syner-gistically affected CET pathway with OPPP in N. yezoensis. All the results indicated that changes of FNR content could enhance OPPP, which further promote the CET operation. This is the first time to find the close relationship between photosynthetic electron transportation and carbohydrate metabolism in strong resurrection plant.
关键词	Ferredoxin-NADP plus oxidoreductase Neopyropia yezoensis Oxidized pentose phosphate pathway Cyclic electron transportation
DOI	10.1016/j.algal.2023.103204
收录类别	SCI
语种	英语
资助项目	Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; earmarked fund for CARS, Technology special project in North Jiangsu Province[SZ-LYG202122] ; earmarked fund for CARS, Technology special project in North Jiangsu Province[CARS-50]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:001052196400001
出版者	ELSEVIER
WOS关键词	CYCLIC ELECTRON FLOW ; PHOTOSYSTEM-I ; REDUCTASE FNR ; STRESS ; DEHYDROGENASE ; ASSOCIATION ; DRIVES ; GROWTH ; PGR5
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182799
专题	实验海洋生物学重点实验室
通讯作者	Zheng, Zhenbing; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 168 Wenhai Rd, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Guo, Menglin,Shao, Zhizhuo,Zheng, Zhenbing,et al. Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,74:9.
APA	Guo, Menglin,Shao, Zhizhuo,Zheng, Zhenbing,&Wang, Guangce.(2023).Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,74,9.
MLA	Guo, Menglin,et al."Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 74(2023):9.