Comparative proteomic and phosphoproteomic analysis reveals differential heat response mechanism in two congeneric oyster species

doi:10.1016/j.ecoenv.2023.115197

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative proteomic and phosphoproteomic analysis reveals differential heat response mechanism in two congeneric oyster species
	Wang, Chaogang 1,2,3; Du, Mingyang 1,2,3; Jiang, Zhuxiang 1,2,3; Cong, Rihao 1,2,6,7; Wang, Wei 1,5,6,7; Zhang, Guofan 1,2,4,6,7; Li Acdefg, Li 1,3,4,5,6,7
	2023-09-15
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	263 页码:19
通讯作者	Li Acdefg, Li(lili@qdio.ac.cn)
摘要	High-temperature stress caused by global climate change poses a significant threat to marine ectotherms. This study investigated the role of protein phosphorylation modifications in the molecular regulation network under heat stress in oysters, which are representative intertidal organisms that experience considerable temperature changes. Firstly, the study compared the extent of thermal damage between two congeneric oyster species, the relative heat-tolerant Crassostrea angulata (C. angulata) and heat-sensitive Crassostrea gigas (C. gigas), under sublethal temperature (37 degrees C) for 12 h, using various physiological and biochemical methods. Subsequently, the comparative proteomic and phosphoproteomic analyses revealed that high-temperature considerably regulated signal transduction, energy metabolism, protein synthesis, cell survival and apoptosis, and cytoskeleton remodeling through phosphorylation modifications of related receptors and kinases. Furthermore, the protein kinase A, mitogen-activated protein kinase 1, tyrosine-protein kinase Src, and serine/threonine kinase AKT, exhibiting differential phosphorylation modification patterns, were identified as hub regulators that may enhance glycolysis and TCA cycle to increase the energy supply, distribute protein synthesis, inhibit Caspasedependent apoptosis activated by endogenous mitochondrial cytochrome release and maintain cytoskeletal stability, ultimately shaping the higher thermal resistance of C. angulata. This study represents the first investigation of protein phosphorylation dynamics in marine invertebrates under heat stress, reveals the molecular mechanisms underlying the differential thermal responses between two Crassostrea oysters at the phosphorylation level, and provides new insights into understanding phosphorylation-mediated molecular responses in marine organisms during environmental changes and predicting the adaptive potential in the context of global warming.
关键词	Heat stress Oysters Crassostrea angulata Crassostrea gigas Phosphorylation
DOI	10.1016/j.ecoenv.2023.115197
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:001044629800001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	NF-KAPPA-B ; AFFINITY-REGULATING KINASE ; ARGININE PHOSPHORYLATION ; PROTEIN-PHOSPHORYLATION ; INTERMEDIATE-FILAMENTS ; ENVIRONMENTAL-STRESS ; MOLECULAR-CLONING ; SUMMER MORTALITY ; GENE-EXPRESSION ; THERMAL-STRESS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182730
专题	实验海洋生物学重点实验室
通讯作者	Li Acdefg, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan, Peoples R China 5.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China 7.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Chaogang,Du, Mingyang,Jiang, Zhuxiang,et al. Comparative proteomic and phosphoproteomic analysis reveals differential heat response mechanism in two congeneric oyster species[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,263:19.
APA	Wang, Chaogang.,Du, Mingyang.,Jiang, Zhuxiang.,Cong, Rihao.,Wang, Wei.,...&Li Acdefg, Li.(2023).Comparative proteomic and phosphoproteomic analysis reveals differential heat response mechanism in two congeneric oyster species.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,263,19.
MLA	Wang, Chaogang,et al."Comparative proteomic and phosphoproteomic analysis reveals differential heat response mechanism in two congeneric oyster species".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 263(2023):19.