Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus

doi:10.1016/j.cbpb.2023.110848

IOCAS-IR > 实验海洋生物学重点实验室

	Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus
	Zou, Congcong1,2,3 ; Wang, Wenxiang 1,2,3; Shu, Chang1,2,3 ; Liang, Shaoshuai 1,2; Zou, Yuxia 1,2; Wang, Lijuan1,2 ; Wu, Zhihao 1,2; Liu, Yan1,2 ; You, Feng 1,2
	2023-06-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	266 页码:10
通讯作者	You, Feng(youfeng@qdio.ac.cn)
摘要	Steroidogenesis is an important biological process for gonadal differentiation and development. In mammals, 38hydroxysteroid dehydrogenase 7 (HSD3B7) could convert 38-hydroxy of 7 & alpha;-hydroxycholesterol into a ketone and form 7 & alpha;-hydroxy-4-cholesten-3-one, which may affect steroidogenesis. However, in fish, the study of Hsd3b7 is still lacking. In this study, Hsd3b7 was identified in the olive flounder Paralichthys olivaceus, an important mariculture fish. According to bioinformatics analysis, Hsd3b7 belongs to a Rossmann-fold NAD(P)(+)-binding protein and can interact in a predictable manner with Hsd17b2, -3, and - 4, which play a role in steroidogenesis. In the adult flounder, Hsd3b7 was expressed in various tissues, at particularly high level in male muscle. The expression levels of Hsd3b7 at gonadal development stages I-V initially increased and then decreased, with an inflection point in the ovary at stage III and in the testis at stage IV. At stage III, the expression level of Hsd3b7 was significantly higher in the ovary than in the testis (P < 0.01). The results of in situ hybridization (ISH) revealed that it was mainly expressed in oocytes of phases I-IV or around oocytes of phases IV-V in the ovaries and around spermatid lobules at stages IV-V in the testes. Three regulatory sites of SRY-box transcription factor 9 (Sox9), a transcription factor involved in steroidogenesis and gonadal differentiation, were predicted in the promoter of Hsd3b7. After intraperitoneal injection with the recombination flounder Sox9a, the expression of Hsd3b7 was significantly up-regulated (P < 0.01). During the flounder gonadal differentiation, 178-estradiol (E2, 5 & mu;g/g feed) and 17 & alpha;-methyltestosterone (T, 5 & mu;g/g feed) were used to obtain the phenotypic female or male flounder, and the results showed that in the E2 group, Hsd3b7 expression was highest at 2 cm TL, the primordial gonad stage, which was significantly higher than that at 12 cm TL (P < 0.05). In the T group, Hsd3b7 expression level was also highest at 2 cm TL and significantly higher than at 10 and 12 cm TL (P < 0.05). Moreover, Hsd3b7 was detected to be localized mainly around oogonia and spermatogonia during the differentiated gonads with ISH. These findings first introduce the expression characteristics of Hsd3b7 and the effect of Sox9a on its expression, which contribute to our understanding of the function of Hsd3b7 in fish gonads.
关键词	Flounder Hsd3b7 Expression Gonadal differentiation and development Sox9a
DOI	10.1016/j.cbpb.2023.110848
收录类别	SCI
语种	英语
资助项目	National Key Research and Devel- opment Program of China[2022YFD2400402] ; National Key Research and Devel- opment Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31602143] ; National Natural Science Foundation of China[31772834] ; National Marine Genetic Resource Center
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
WOS类目	Biochemistry & Molecular Biology ; Zoology
WOS记录号	WOS:001041123500001
出版者	ELSEVIER SCIENCE INC
WOS关键词	DIFFERENTIATION ; HORMONE ; TEMMINCK
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182724
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zou, Congcong,Wang, Wenxiang,Shu, Chang,et al. Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2023,266:10.
APA	Zou, Congcong.,Wang, Wenxiang.,Shu, Chang.,Liang, Shaoshuai.,Zou, Yuxia.,...&You, Feng.(2023).Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,266,10.
MLA	Zou, Congcong,et al."Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 266(2023):10.