The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean

doi:10.1007/s00248-023-02284-w

	The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean
	Jiang, Zhichen1,2,3 ; Liu, Sizhen 4,5; Zhang, Dechao 1,2,3; Sha, Zhongli1,2,3
	2023-08-08
发表期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
页码	9
通讯作者	Zhang, Dechao(zhangdechao@qdio.ac.cn)
摘要	To better understand bacterial communities and metabolism under nitrogen deficiency, 154 seawater samples were obtained from 5 to 200 m at 22 stations in the photic zone of the Western North Pacific Ocean. Total 634 nitrate-utilizing bacteria were isolated using selective media and culture-dependent methods, and 295 of them were positive for nitrate reduction. These nitrate-reducing bacteria belonged to 19 genera and 29 species and among them, Qipengyuania flava, Roseibium aggregatum, Erythrobacter aureus, Vibrio campbellii, and Stappia indica were identified from all tested seawater layers of the photic zone and at almost all stations. Twenty-nine nitrate-reducing strains representing different species were selected for further the study of nitrogen, sulfur, and carbon metabolism. All 29 nitrate-reducing isolates contained genes encoding dissimilatory nitrate reduction or assimilatory nitrate reduction. Six nitrate-reducing isolates can oxidize thiosulfate based on genomic analysis and activity testing, indicating that nitrate-reducing thiosulfate-oxidizing bacteria exist in the photic zone. Five nitrate-reducing isolates obtained near the chlorophyll a-maximum layer contained a dimethylsulfoniopropionate synthesis gene and three of them contained both dimethylsulfoniopropionate synthesis and cleavage genes. This suggests that nitrate-reducing isolates may participate in dimethylsulfoniopropionate synthesis and catabolism in photic seawater. The presence of multiple genes for chitin degradation and extracellular peptidases may indicate that almost all nitrate-reducing isolates (28/29) can use chitin and proteinaceous compounds as important sources of carbon and nitrogen. Collectively, these results reveal culturable nitrate-reducing bacterial diversity and have implications for understanding the role of such strains in the ecology and biogeochemical cycles of nitrogen, sulfur, and carbon in the oligotrophic marine photic zone.
关键词	Diversity Nitrate-reducing bacteria The photic zone Pacific Ocean
DOI	10.1007/s00248-023-02284-w
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050302] ; National Natural Science Foundation of China[41976166] ; National Natural Science Foundation of China[42025603] ; Marine Samp;T Fund of Shandong Province for Qingdao Marine Science and Technology enter[2022QNLM030004-3] ; Marine Samp;T Fund of Shandong Province for Qingdao Marine Science and Technology enter[LSKJ202203100] ; NSFC Shiptime Sharing Project[42249901]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
WOS类目	Ecology ; Marine & Freshwater Biology ; Microbiology
WOS记录号	WOS:001044333900001
出版者	SPRINGER
WOS关键词	NITROGEN-CYCLE ; SP. NOV. ; MARINE ; COMMUNITIES ; REDUCTION ; GENE ; SEA ; REDUCTASES ; ABUNDANCES ; STAPPIA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182718
专题	海洋生物分类与系统演化实验室
通讯作者	Zhang, Dechao
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China 2.Laoshan Lab, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China 5.Huazhong Agr Univ, Coll Informat, Hubei Key Lab Agr Bioinformat, Wuhan 430070, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jiang, Zhichen,Liu, Sizhen,Zhang, Dechao,et al. The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean[J]. MICROBIAL ECOLOGY,2023:9.
APA	Jiang, Zhichen,Liu, Sizhen,Zhang, Dechao,&Sha, Zhongli.(2023).The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean.MICROBIAL ECOLOGY,9.
MLA	Jiang, Zhichen,et al."The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean".MICROBIAL ECOLOGY (2023):9.