Innovative Eco-Friendly Microwave-Assisted Rapid Biosynthesis of Ag/AgCl-NPs Coated with Algae Bloom Extract as Multi-Functional Biomaterials with Non-Toxic Effects on Normal Human Cells

doi:10.3390/nano13142141

IOCAS-IR > 实验海洋生物学重点实验室

	Innovative Eco-Friendly Microwave-Assisted Rapid Biosynthesis of Ag/AgCl-NPs Coated with Algae Bloom Extract as Multi-Functional Biomaterials with Non-Toxic Effects on Normal Human Cells
	Pekkoh, Jeeraporn 1,2,3; Ruangrit, Khomsan 2; Kaewkod, Thida 1; Tragoolpua, Yingmanee 1; Hoijang, Supawitch 4; Srisombat, Laongnuan 4; Wichapein, Antira 1,5; Pathom-aree, Wasu 1,5; Kato, Yasuo 6,7; Wang, Guangce 8,9; Srinuanpan, Sirasit 1,5
	2023-07-01
发表期刊	NANOMATERIALS
卷号	13 期号:14 页码:27
通讯作者	Srinuanpan, Sirasit(sirasit.s@cmu.ac.th)
摘要	Harmful algal blooms impact human welfare and are a global concern. Sargassum spp., a type of algae or seaweed that can potentially bloom in certain regions of the sea around Thailand, exhibits a noteworthy electron capacity as the sole reducing and stabilizing agent, which suggests its potential for mediating nanoparticle composites. This study proposes an eco-friendly microwave-assisted biosynthesis (MAS) method to fabricate silver nanoparticles coated with Sargassum aqueous extract (Ag/AgCl-NPs-ME). Ag/AgCl-NPs-ME were successfully synthesized in 1 min using a 20 mM AgNO3 solution without additional hazardous chemicals. UV-visible spectroscopy confirmed their formation through a surface plasmon resonance band at 400-500 nm. XRD and FTIR analyses verified their crystalline nature and involvement of organic molecules. TEM and SEM characterization showed well-dispersed Ag/AgCl-NPs-ME with an average size of 36.43 nm. The EDS results confirmed the presence of metallic Ag+ and Cl- ions. Ag/AgCl-NPs-ME exhibited significant antioxidant activity against free radicals (DPPH, ABTS, and FRAP), suggesting their effectiveness. They also inhibited enzymes (tyrosinase and ACE) linked to diseases, indicating therapeutic potential. Importantly, the Ag/AgCl-NPs-ME displayed remarkable cytotoxicity against cancer cells (A375, A549, and Caco-2) while remaining non-toxic to normal cells. DNA ladder and TUNEL assays confirmed the activation of apoptosis mechanisms in cancer cells after a 48 h treatment. These findings highlight the versatile applications of Ag/AgCl-NPs-ME in food, cosmetics, pharmaceuticals, and nutraceuticals.
关键词	anticancer antioxidant biosynthesis macroalgae microwave silver nanoparticles
DOI	10.3390/nano13142141
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS记录号	WOS:001036727100001
出版者	MDPI
WOS关键词	SILVER NANOPARTICLES AGNPS ; GREEN SYNTHESIS ; ANTIOXIDANT ACTIVITY ; AQUEOUS EXTRACT ; DEGRADATION ; ANTIBACTERIAL ; TOXICITY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182578
专题	实验海洋生物学重点实验室
通讯作者	Srinuanpan, Sirasit
作者单位	1.Chiang Mai Univ, Fac Sci, Dept Biol, Chiang Mai 50200, Thailand 2.Chiang Mai Univ, Multidisciplinary Res Inst, Chiang Mai 50200, Thailand 3.Chiang Mai Univ, Fac Sci, Environm Sci Res Ctr, Chiang Mai 50200, Thailand 4.Chiang Mai Univ, Fac Sci, Dept Chem, Chiang Mai 50200, Thailand 5.Chiang Mai Univ, Ctr Excellent Microbial Divers & Sustainable Utili, Chiang Mai 50200, Thailand 6.Toyama Prefectural Univ, Biotechnol Res Ctr, Toyama 9390398, Japan 7.Toyama Prefectural Univ, Dept Biotechnol, Toyama 9390398, Japan 8.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 9.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Pekkoh, Jeeraporn,Ruangrit, Khomsan,Kaewkod, Thida,et al. Innovative Eco-Friendly Microwave-Assisted Rapid Biosynthesis of Ag/AgCl-NPs Coated with Algae Bloom Extract as Multi-Functional Biomaterials with Non-Toxic Effects on Normal Human Cells[J]. NANOMATERIALS,2023,13(14):27.
APA	Pekkoh, Jeeraporn.,Ruangrit, Khomsan.,Kaewkod, Thida.,Tragoolpua, Yingmanee.,Hoijang, Supawitch.,...&Srinuanpan, Sirasit.(2023).Innovative Eco-Friendly Microwave-Assisted Rapid Biosynthesis of Ag/AgCl-NPs Coated with Algae Bloom Extract as Multi-Functional Biomaterials with Non-Toxic Effects on Normal Human Cells.NANOMATERIALS,13(14),27.
MLA	Pekkoh, Jeeraporn,et al."Innovative Eco-Friendly Microwave-Assisted Rapid Biosynthesis of Ag/AgCl-NPs Coated with Algae Bloom Extract as Multi-Functional Biomaterials with Non-Toxic Effects on Normal Human Cells".NANOMATERIALS 13.14(2023):27.