Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei | |
Si, Shuqing1,2,3; Zhang, Xiaojun1,2,3,4,5,6; Yu, Yang1,2,4,5,6 ; Zhang, Xiaoxi1,2,4,5; Zhong, Xiaoyun1,2,6; Yuan, Jianbo1,2,4,5,6; Yang, Song3; Li, Fuhua1,2,4,5,6
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| 2023-07-07 | |
| Source Publication | FRONTIERS IN GENETICS
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| Volume | 14Pages:16 |
| Corresponding Author | Zhang, Xiaojun(xjzhang@qdio.ac.cn) |
| Abstract | Monocyte to macrophage differentiation factor 2 gene (Mmd2) encodes a member of the progestin and adipoQ receptor (PAQR) family, and plays a key role in growth and development. Our previous studies had found Mmd2 (Monocyte to macrophage differentiation factor 2) is a new candidate gene for growth traits in Pacific white shrimp (Litopenaeus vannamei). For the purpose of understanding the underlying mechanism of LvMmd2 affecting the growth of shrimp, we analyzed the gene structure, phylogeny, expression profiles and RNA interference of this gene in L. vannamei. We found the LvMmd2 gene sequence was highly conserved in transmembrane regions, it was widely expressed in different tissues, with the highest expression level in the eye stalk. Knockdown LvMmd2 could significantly promote body length and body weight gain, suggesting it is a growth suppressor. Through transcriptome analysis we identified 422 differentially expressed genes (DEGs) between the dsMmd2 group and control group, among which 337 genes were upregulated in the dsMmd2 group, including numerous muscle-related genes and protein synthesis genes. Further bioinformatics analysis showed that growth, metabolism, and immune-related signal pathway had changed significantly. The above results greatly increase our understanding on the conservative structure and function of LvMmd2 gene, and provide potential application prospects in genetics and breeding. |
| Keyword | Litopenaeus vannamei MMD2 PAQR10 gene structures expression profile growth |
| DOI | 10.3389/fgene.2023.1151193 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; National Key Ramp;D Program of China[2022YFF1000304] ; National Key Ramp;D Program of China[2018YFD0900103] ; National Natural Sciences Foundation of China[31972782] ; National Natural Sciences Foundation of China[32273102] |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:001034180300001 |
| Publisher | FRONTIERS MEDIA SA |
| WOS Keyword | WNT/BETA-CATENIN ; GROWTH ; IDENTIFICATION ; EXPRESSION ; ACTIVATION ; HORMONE ; CLONING ; BINDING ; ALPHA ; SOX9 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/182542 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Zhang, Xiaojun |
| Affiliation | 1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Agr Univ, Sch Life & Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Si, Shuqing,Zhang, Xiaojun,Yu, Yang,et al. Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei[J]. FRONTIERS IN GENETICS,2023,14:16. |
| APA | Si, Shuqing.,Zhang, Xiaojun.,Yu, Yang.,Zhang, Xiaoxi.,Zhong, Xiaoyun.,...&Li, Fuhua.(2023).Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei.FRONTIERS IN GENETICS,14,16. |
| MLA | Si, Shuqing,et al."Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei".FRONTIERS IN GENETICS 14(2023):16. |
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