Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei

doi:10.3389/fgene.2023.1151193

IOCAS-IR > 实验海洋生物学重点实验室

	Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei
	Si, Shuqing 1,2,3; Zhang, Xiaojun 1,2,3,4,5,6; Yu, Yang1,2,4,5,6 ; Zhang, Xiaoxi1,2,4,5 ; Zhong, Xiaoyun 1,2,6; Yuan, Jianbo 1,2,4,5,6; Yang, Song 3; Li, Fuhua 1,2,4,5,6
	2023-07-07
发表期刊	FRONTIERS IN GENETICS
卷号	14 页码:16
通讯作者	Zhang, Xiaojun(xjzhang@qdio.ac.cn)
摘要	Monocyte to macrophage differentiation factor 2 gene (Mmd2) encodes a member of the progestin and adipoQ receptor (PAQR) family, and plays a key role in growth and development. Our previous studies had found Mmd2 (Monocyte to macrophage differentiation factor 2) is a new candidate gene for growth traits in Pacific white shrimp (Litopenaeus vannamei). For the purpose of understanding the underlying mechanism of LvMmd2 affecting the growth of shrimp, we analyzed the gene structure, phylogeny, expression profiles and RNA interference of this gene in L. vannamei. We found the LvMmd2 gene sequence was highly conserved in transmembrane regions, it was widely expressed in different tissues, with the highest expression level in the eye stalk. Knockdown LvMmd2 could significantly promote body length and body weight gain, suggesting it is a growth suppressor. Through transcriptome analysis we identified 422 differentially expressed genes (DEGs) between the dsMmd2 group and control group, among which 337 genes were upregulated in the dsMmd2 group, including numerous muscle-related genes and protein synthesis genes. Further bioinformatics analysis showed that growth, metabolism, and immune-related signal pathway had changed significantly. The above results greatly increase our understanding on the conservative structure and function of LvMmd2 gene, and provide potential application prospects in genetics and breeding.
关键词	Litopenaeus vannamei MMD2 PAQR10 gene structures expression profile growth
DOI	10.3389/fgene.2023.1151193
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; National Key Ramp;D Program of China[2022YFF1000304] ; National Key Ramp;D Program of China[2018YFD0900103] ; National Natural Sciences Foundation of China[31972782] ; National Natural Sciences Foundation of China[32273102]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001034180300001
出版者	FRONTIERS MEDIA SA
WOS关键词	WNT/BETA-CATENIN ; GROWTH ; IDENTIFICATION ; EXPRESSION ; ACTIVATION ; HORMONE ; CLONING ; BINDING ; ALPHA ; SOX9
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182542
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Xiaojun
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Agr Univ, Sch Life & Sci, Qingdao, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Si, Shuqing,Zhang, Xiaojun,Yu, Yang,et al. Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei[J]. FRONTIERS IN GENETICS,2023,14:16.
APA	Si, Shuqing.,Zhang, Xiaojun.,Yu, Yang.,Zhang, Xiaoxi.,Zhong, Xiaoyun.,...&Li, Fuhua.(2023).Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei.FRONTIERS IN GENETICS,14,16.
MLA	Si, Shuqing,et al."Structure and function analyses of the Mmd2 gene in pacific white shrimp Litopenaeus vannamei".FRONTIERS IN GENETICS 14(2023):16.