Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED

doi:10.1016/j.aqrep.2023.101638

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED
	Wang, Guoyu 1,2,3; Wu, Zhihao 2,3; Wang, Lijuan2,3 ; Xiong, Yuyu 4; You, Feng 2,3
	2023-08-01
发表期刊	AQUACULTURE REPORTS
ISSN	2352-5134
卷号	31 页码:16
通讯作者	Wu, Zhihao(wuzhihao@qdio.ac.cn) ; You, Feng(youfeng@qdio.ac.cn)
摘要	Light spectrum is an important environmental factor affecting fish activities. However, the existing studies on spectrum are usually conducted under monochromatic light, which are multiple variables compared to the natural light conditions and increase uncertainty. The results may be caused by the absence of all other chromatic lights. The tiger puffer Takifugu rubripes is an important commercial marine fish in China, Japan, and Korea. In this study, the effects of red light on the eyes and brain of T. rubripes brooder, including histological changes in retina and optic tectum, the levels of oxidative stress indicators in serum, and changes in gene expression at transcriptome level were examined, after the treatment of full spectrum LED (Fs) and red light filtered LED (Rf) for 52 days. The results showed that the growth and histological structure of retina and optic tectum were not significantly altered. While, Rf significantly decreased the activity of superoxide dismutase and catalase, and increased the content of cortisol (P < 0.05), but had no effect on the levels of glutathione peroxidase and malondialdehyde. Transcriptome data revealed that there were 147 differentially expressed genes (DEGs) in the eyes of Rf vs Fs. Many DEGs related to vision changed significantly. KEGG enrichment and GSEA analyses showed that the significantly enriched arachidonic acid pathway down-regulated in the Rf group, in which genes related to sexual maturation and ovulation presented significant changes, suggesting that Rf may affect the ovulation process through this pathway. In the brain, the number of DEGs was only 20, which are most correlated with circadian rhythm. The present study displayed for the first time the role of red light in promoting ovulation in mature fish by filtering it, which may provide more supports to highlight the function of red light in fish culture.
关键词	Puffer Red light filtered LED Eyes and brain Oxidative stress index Transcriptome
DOI	10.1016/j.aqrep.2023.101638
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2019YFD0900503] ; National Natural Science Foundation of China[31772834] ; National Marine Genetic Resource Center
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:001030387700001
出版者	ELSEVIER
WOS关键词	OXIDATIVE STRESS ; ARACHIDONIC-ACID ; GROWTH ; SPECTRUM ; PERFORMANCE ; EXPRESSION ; MATURATION ; CLOWNFISH ; OVULATION ; BEHAVIOR
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182536
专题	实验海洋生物学重点实验室
通讯作者	Wu, Zhihao; You, Feng
作者单位	1.Qingdao Agr Univ, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Guoyu,Wu, Zhihao,Wang, Lijuan,et al. Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED[J]. AQUACULTURE REPORTS,2023,31:16.
APA	Wang, Guoyu,Wu, Zhihao,Wang, Lijuan,Xiong, Yuyu,&You, Feng.(2023).Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED.AQUACULTURE REPORTS,31,16.
MLA	Wang, Guoyu,et al."Comparative study of transcriptome profiles in the eyes and brain of the tiger puffer Takifugu rubripes brooder under red light filtered LED".AQUACULTURE REPORTS 31(2023):16.